Experimental Study Of Mannitol Enhanced Catharsis On Relieving Liver Injury Induced By A.subpallidorosea Poisoning Poisoning

Objective: To investigate whether mannitol enhanced catharsis can reduce liver injury caused by A.subpallidorosea poisoning in rabbits.Methods: Twenty-four normal healthy male rabbits,aged about 8 months and weighing 2±0.2kg,were randomly assigned to blank control group,mannitol control group,poisoning model group and mannitol treatment group.Blank control group: at the same time point as the poisoning model group,20 ml normal saline was given to the stomach for 3 times/day;mannitol control group: 20%mannitol 20 ml was administered 3 times/day at the same time point as the mannitol treatment group;poisoning model group: the prepared extract of dried fruiting body of A.subpallidorosea was administered by one-time gavage,the dose was 0.1g(dried fruiting body of A.subpallidorosea)/kg,and the normal saline was administered 3 times/day after 6 hours;mannitol treatment group:20% mannitol 20 ml was administered 3 times/day after poisoned 6 hours.After72 hours of observation,the rabbits were killed.At 0 h,24 h,36 h,48 h and 72 h after gavage of A.subpallidorosea,blood samples were collected from all rabbits' ear marginal arteries for 1 ml to detect Alanine aminotransferase(ALT),total bilirubin(TBIL),direct bilirubin(DBIL),prothrombin time(PT),activated partial thrombin time(APTT)and creatinine(CRE).After the rabbits were killed,the livers were taken for transmission electron microscope(Tem)observation.The general performance score,mortality,liver biochemical indexes(total bilirubin,direct bilirubin and alanine aminotransferase),thrombin function(prothrombin time and activated partial thrombin time),renal function(creatinine)and liver transmission electron microscopy were observed.Results: 1.General performance and death evaluation of rabbits: the appetite,activity,sclera,urination and survival of rabbits in the poisoning model group and the mannitol treatment group were worse than those in the blank control group(all P < 0.01).After mannitol drainage treatment,the general performance and death of rabbits in the treatment group were better than those in the poisoning model group(all P < 0.01).There was no significant difference between the mannitol control group and the blank control group.2.The liver function and coagulation function of rabbits in the poisoning model group increased after mold making,in which ALT,PT and APTT reached the peak at 36 hours after mold making [ ALT(1526.7±53.6U/L);PT(82.1±7.2 s);APTT(132.5±9.6 s)],and then gradually decreased.TBIL peaked 48 hours after molding [ TBIL(52.5±4.8umol/L);DBIL(23.56±4.43umol/L)] and then gradually declined.Compared with the blank control group,ALT,PT,APTT,TBIL and DBIL in the poisoning model group were significantly increased(all P < 0.05).After mannitol drainage treatment,the indexes of liver function and coagulation function in the mannitol treatment group decreased(all P < 0.05),but were stillsignificantly higher than those in the blank control group(all P < 0.05).There was no significant difference in liver function and coagulation function between the mannitol control group and the blank control group.Creatinine levels were normal in each group.3.Tem examination: the liver cells in the poisoning model group were seriously damaged compared with the blank control group.In the poisoning model group,the liver nuclei were wrinkled,the cell structure was unclear,and the organelles were seriously damaged,while the damage degree was reduced in the mannitol treatment group.Conclusion: Mannitol enhanced catharsis can reduce the liver damage of rabbits poisoned by A.subpallidorosea.