| Objective:Metabolomics analysis was performed on the urine of 14 patients with early,17 patients with advanced and 13 healthy patients with colon cancer using liquid chromatography-mass spectrometry(lc-cs),looking for a landmark in the metabolic production of early colorectal cancer,to early diagnosis and screening of tumor contrast differences between patients with colon cancer patients and healthy subjects metabolites,expect to get meaningful difference product,to explain the pathogenesis of colorectal cancerMethods: lc-ms method was used to analyze the collected urine of patients with early advanced colorectal cancer and urine of healthy people.The obtained original data were analyzed by a variety of statistical tools and methods.Statistical methods such as principal component analysis,discriminant analysis and cluster analysis were used to find representative differential metabolitesResult: Through lc-ms analysis,principal component analysis,variance analysis,partial minimization analysis,support vector discriminant analysis,cluster analysis,ROC curve drawing and other analysis methods,the following results are obtained: 1.4 statistically significant metabolites were obtained between the urine of colorectal cancer patients and that of healthy patients,including phosphatidylcholine,Sphinganine,Stigmastanol,and cardiolipin.2.Three statistically significant metabolites were obtained from the samples of patients and healthy patients with early colorectal cancer,namely sphingaminol,Stigmastanol,and cardiolipin.Conclusion:Sphingosinol and its metabolites have significant differences between patients with early colorectal cancer and healthy people,which may be a potential marker for early diagnosis of colorectal cancer.Sphingosinol,sterol,and cardiolipin are significantly different in the urine concentrations of colorectal cancer patients and healthy people,which may be a potential marker for extensive screening of colorectal cancer. |
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