Anti-hypoxia Effect And Mechanism Study Of Potentilla Anserina L Polysaccharide

In recent years,due to the activities of military,the implementation of the strategy for large-scale development of western China,the development of tourism,the convenience of transportation and other factors,more and more people have entered high altitude area.Many people suffer from a series of plateau diseases,such as High altitude cerebral edema(HACE)and High altitude pulmonary edema(HAPE).HACE and HAPE are potential life-threating illness as its acute onset,rapid progress and high mortality.Currently,there are many drugs to treat the above two diseases,but all have side effects.Thus,researchers' attention has been transformed into plant polysaccharides of natural origin,which have green and safe characteristics.Potentilla anserina L.is a unique dual-purpose plant that can be used for food and medicine in plateau area,which is rich in polysaccharide and has high medicinal and nutritional value.In this paper,the therapeutic effect of Potentilla anserina L polysaccharide on high altitude cerebral edema and high altitude pulmonary edema was studied,and its mechanism was studied preliminarily.The research content is as follows:(1)The polysaccharide with best anti-hypoxia effect were selected from Potentilla anserina polysaccharide(PAP),Cynomorium songarieum polysaccharide(CSRP),Sphallerocarpus gracilis polysaccharide(SGP)and Lilium Brownii polysaccharide(LP)by normobaric hypoxia and acute hypoxia experiments in mice.Then explore the dose-effect relationship of dominant polysaccharides.The experimental results showed that the anti-hypoxia effects of CSRP,SGP and LP were all significant,but the anti-hypoxia effect of PAP was the most significant.Therefore,in this study,PAP was selected as the main research object.According to the results of the dose-response relationship of PAP to hypoxia,the anti-hypoxia effect of PAP in the low(150 mg/kg),medium(300 mg/kg)and high(500 mg/kg)dose groups are dose-dependent,so the above dose are considered as the reference dose for the next experiment.(2)Establish HACE and HAPE rat models,and randomly divide the rats into normoxia control group(NC),hypoxia model(HHM)group,PAP-L group(100 mg/kg),PAP-M group(200 mg/kg),PAP-H group(400 mg/kg),Dex group(4 mg/kg).Observe the survival status of the rats during establish model.After sacrifice,measure the water content of brain tissue and lung tissue by dry and wet specific gravity method.Observe the pathological changes of brain tissue and lung tissue by HE staining.The antioxidative stress capacity of PAP was also evaluated by the contents of MDA,NO,SOD and GSH.Finally,the protein content and mRNA expression level of pro-inflammatory cytokines(IL-1?,IL-6,TNF-?,VEGF,NF-?B and HIF-1?)in brain and lung tissue were measured by ELISA,RT-PCR and Western Blotting.The experimental results show that PAP reduces the water content in the brain and lung tissues.Ameliorate the damage to brain and lung tissues.Reduces the content of MDA and NO,and increases the activity of SOD and GSH.In addition,PAP blocking the NF-?B and HIF-1? signaling pathway activation inhibited the generation of downstream pro-inflammatory cytokines(IL-1?,IL-6,TNF-? and VEGF).Therefore,PAP has a potential to treat and prevent of HACE by suppression of oxidative stress and inflammatory response.