| Objective: Using 6Me V and 8Me V electron beams to accurately irradiate normal gastric tissues of SD rats at a single 20 Gy to simulate gastric injury caused by intraoperative radiotherapy.Observing and comparing the gastric injury and repair of rats after two kinds of energy irradiation,and provide experimental basis for the reasonable selection of energy and dose in the intraoperative radiotherapy of clinical gastric cancer and the prevention and treatment of radiation gastric injury.Method: Dividing seventy two healthy male twelve-week-old SD rats into three groups:control group C(8 rats),experimental group A(32 rats)and experimental group B(32 rats).The stomach of the rats in the experimental group A and B were subject to single 6Me V 20 Gy and 8Me V 20 Gy irridiation by using the instructor's patented technology "Small animal precise radiation test-bed" with 3cm x 3cm Irradiated wild lead,and the irradiation field was 3cm×3cm,Gastric injury and repairment were observed at the 14 th,28th,42 th,56th day following irradiation.Results: After the 6Me V electron ray 20 Gy irradiation,the rats in group A had sparse fur and dry skin.The dietary amount was less than that of the control group within 8 days(P <0.05),and there was no significant difference after the 12 th day between group A and control group(P> 0.05);In the first 14 days,the rats' activity was reduced and their spirits were diminished.After 28 days,their activities and mental state improved,and on the 56 th day,they were close to the control group.Experimental group B rats were irradiated with 8Me V electron beams 20 Gy irradiation,and the hair loss and dryness of the exposed area were heavier than those of group A;the dietary amount was less than that of the control group and group A within 12 days(P <0.05).There was no significant difference after the 16 th day comparing with the control group and group A(P> 0.05),and the mental state changes were similar to that of group A.In the control group C,the skin was soft,the fur was thick and smooth,and it was lively and active;the amount of diet increased relatively steadily.Before irradiation: there was no significant difference in body weight between the control group C,the experimental group A and the experimental group B(P>0.05);after irradiation: the body weight of the two experimental groups showed a trend of decreasing and then increasing.The weight of the control group has been growing relatively steadily.The weight of the experimental group A was lower than that of the control group on days 7,14,21,28,35,42,and 49(P<0.05),and was close to the control group on day 56(P>0.05).The weight of the experimental group B has been slightly lower than that of the control group and the experimental group A.There was a statistical difference between the group B and control group within 56 days(P <0.05).On the 56 th day,the weight of group B was still lower than that of the control group(P<0.05).On day 14?21?28,35,42,49,the difference between group A and B was statistically significant(P<0.05).General pathology:No gastric perforation was found in the experimental group A and the experimental group B within 56 days.In the experimental group A:On the 14 th day,the surface of the stomach was fibrotic and adhered to the surrounding tissues,which was bright red;on the 28 th day,the color was gray-red,and adhered closely with surrounding tissue;to the 56 th day,adhesion is tight,and the color changes to grayish yellow.In the experimental group B:On the 14 th day,Gastric congestion and adhesion to surrounding tissues were heavier than group A.On the 28 th day,the gastric tissues were still congested,edema.On the 56 th day,the color gradually changed to gray-yellow,and fibrous adhesion was heavier than the control group and the group A.In Group C,there was no hyperemia,Edema and fibrosis,and no adhesion with the surrounding tissues.HE staining examination: On the 14 th day after irradiation,mucosal erosion and submucosal edema were seen microscopically in the experimental group A,and inflammatory cell infiltration was visible in the mucosa and submucosa.The above phenomenon in the experimental group B was worse than that in the group A.On the 28 th day,Submucosal edema in group A is still obvious,fibrosis was seen in submucosa,and glandular atrophy was significantly worse than before.It was the main manifestation under the microscope,and inflammatory cell infiltration increased.Inflammatory cell infiltration,glandular atrophy,and fibrosis in the group B were more significant than that in the group A,and submucosal edema was worse than before.On the 42 th day,mucosal erosions in both experimental groups were basically repaired,and no glandular atrophy was recovered.Submucosal edema was not obvious in group A,fibrosis was worsened than before,and inflammatory cell infiltration was reduced than before.Group B still had submucosal edema,but was less than before.Fibrosis was more significant than group A,and inflammatory cell infiltration was still obvious.On the 56 th day,the mucosal erosion and submucosal edema in both experimental groups were basically repaired,and the inflammatory cell infiltration was significantly reduced than before.The microscopic manifestations were glandular atrophy and fibrosis.Group C had smooth Mucosa without loss,no edema,fibrosis and gland atrophy.Conclusion: 1.The stomach of SD rats can tolerate single 20 GY irradiation of 6Me V and 8Me V electron wires,and there is no serious complication such as perforation;2.At the same time after irradiation,the stomach injury of rats in the 8Me V group was more serious than that of rats in the 6Me V group,and the recovery time was longer.whether it can tolerate higher energy and dose of electron irradiation needs further study. |
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