Expression Of Activated T-cell Nuclear Factor In Patients With Different Levels Of Hypertension In Xinjiang Kazakh

Objective: To study the expression level of nuclear factor of activated T cells(NFAT)and its related molecules in peripheral blood T-lymphocytes of different grade hypertension patients in Xinjiang Kazakh.Methods: From October 2018 to June 2019,30 Kazakh hypertensive patients without drug intervention in the hypertension ward of the First Affiliated Hospital of Xinjiang Medical University were selected as the experimental group,and 30 Kazakh healthy people with normal blood pressure were selected as the control group,with equal proportions of men and women in all groups.Peripheral blood was collected from patients who met the requirements.T-lymphocytes were isolated by immunomagnetic beads.The gene and protein expression of NFAT were detected by RT-q PCR and Western blotting.The expression of IL-17 m RNA was detected by RT-q PCR.Detection of serum IL-17 by ELISA.Results: 1)?NFATm RNA expression levels were positively correlated with hypertension grade 2 and hypertension grade 3(P< 0.01);2)?NFAT protein and IL-17 m RNA expression levels were positively correlated with hypertension grade 3(P< 0.05);3)?The relative expression of NFAT m RNA,protein and IL-17 m RNA in grade 3,grade 2 and grade 1 of hypertension was significantly increased compared with the control group(P < 0.05);4)?The expression of IL-17 in hypertension grade 3 and hypertension grade 2 was significantly higher compared with the control group(P < 0.05).Conclusion: The expression of nuclear factor of activated T cells in peripheral blood T-lymphocytes of Xinjiang Kazakh with different grade hypertension increased with the increase of blood pressure level and was positively correlated.