Effect Of MiR-221 Expression On Proliferation,Apoptosis And Invasion Of Thyroid Papillary Carcinoma

Part ?:The expression of miRNA-221 in thyroid papillary carcinomaObjective:To study the expression of miR-221 in thyroid papillary carcinoma(PTC)cells.Methods: The expression of miR-221 was detected by q PCR.Results: The relative expression of miR-221 in papillary thyroid carcinoma was significantly higher than that in adjacent tissues(P < 0.05).Conclusion: The expression of miR-221 is significantly increased in PTC patients.Part ?:Effects of miRNA-221 on proliferation,apoptosis and invasion of thyroid papillary cancer cellsObjective: To investigate the effects of inhibition of miR-221 on PTC cell proliferation,cell cycle,apoptosis rate and cell invasion in vitro.Methods: The expression of miR-221 in thyroid papillary carcinoma K1 cells was detected by real-time fluorescence quantitative RT-PCR,and the cell proliferation was detected by MTT colorimetry,cell cycle and apoptosis rate were analyzed by flow cytometry.Cell migration and invasiveness were detected in Transwell cell.Results: Mi R-221 was significantly expressed in thyroid papillary carcinoma K1 cells.Compared with the negative control group,the growth of K1 cell line was significantly inhibited after miR-221 inhibitor was transfected.Cycle experiment showed that the ratio of G0 / G1 phase in miR-221 inhibitor group increased from 55.87% to 79.65%(P < 0.05)compared with NC group,while the ratio of G2 / M phase decreased from 23.66% to7.23%(P < 0.05).The number of invasive cells in miR-221 high expression group was significantly increased,while in miR-221 inhibitor transfection group was significantly reduced.The average number of invasive cells in miR-221 inhibitor transfection group and NC transfection group was 33.2,50.0,the difference wasstatistically significant(P < 0.05).Conclusion: The expression of miR-221 is significantly increased in PTC cancer cells,while inhibiting the expression of miR-221 can inhibit the proliferation of PTC cell lines,promote the apoptosis of the cell lines and reduce the invasiveness of the cell lines.