| Objectives:In this study,the methods of data mining and network pharmacology were used to study the prescription law of traditional Chinese medicine in the treatment of Ulcerative Colitis(UC)by director Zhou zhenghua,and summarize his clinical experience and academic thoughts on the treatment of UC.The potential target and molecular mechanism of director zhou zhenghua's treatment of UC in combination of basic drugs were preliminarily discussed to provide reference for further experimental research.Research methods:Through the application of TCM inheritance assistant platform software(V2.5)and Microsoft Excel(2016),the medical records of UC patients receiving TCM treatment from September 2017 to December 2019 in the outpatient department of the first affiliated hospital of tianjin university of traditional Chinese medicine,the gastroenterology outpatient department and the ward after being treated by director zhou zhenghua were sorted out and collected.Screening was conducted in strict accordance with the inclusion criteria and exclusion criteria,data of eligible patients were input into the TCM inheritance auxiliary platform system for analysis and processing,prescription medication law of director zhou zhenghua for treating UC was obtained,and his medication experience and clinical experience were summarized.Under the guidance of zhou's teacher,the basic drug combinations in the above studies were selected,and the network pharmacology method was adopted to collect and screen the active ingredients and targets of traditional Chinese medicine,compare them with UC related targets,and screen the common genes by means of Venn diagram.Cytoscape 3.7.2 software was used to make the "active component-target-disease network map of basic drugs" based on the above data.Based on the PPI database of the protein interaction network,the target interaction network was constructed to extract the candidate target of the basic drug combination therapy for UC,and the GO enrichment analysis and KEGG pathway enrichment analysis were carried out to preliminarily explore the molecular mechanism of the basic drug combination therapy for UCMethods:Through the application of TCM inheritance assistant platform software(V2.5)and Microsoft Excel(2016),the medical records of UC patients receiving TCM treatment from September 2017 to December 2019 in the outpatient department of the first affiliated hospital of tianjin university of traditional Chinese medicine,the gastroenterology outpatient department and the ward after being treated by director zhou zhenghua were sorted out and collected.Screening was conducted in strict accordance with the inclusion criteria and exclusion criteria,data of eligible patients were input into the TCM inheritance auxiliary platform system for analysis and processing,prescription medication law of director zhou zhenghua for treating UC was obtained,and his medication experience and clinical experience were summarized.Under the guidance of zhou's teacher,the basic drug combinations in the above studies were selected,and the network pharmacology method was adopted to collect and screen the active ingredients and targets of traditional Chinese medicine,compare them with UC related targets,and screen the common genes by means of Venn diagram.Cytoscape 3.7.2 software was used to make the"active component-target-disease network map of basic drugs”based on the above data.Based on the PPI database of the protein interaction network,the target interaction network was constructed to extract the candidate target of the basic drug combination therapy for UC,and the GO enrichment analysis and KEGG pathway enrichment analysis were carried out to preliminarily explore the molecular mechanism of the basic drug combination therapy for UC.Results:1 Study on the medication law of Zhou zhenghua in the treatment of ulcerative colitis based on data mining methodThis study collected 103 patients who met the inclusion criteria,with a total of 309 prescriptions.Data were input into the TCM inheritance auxiliary platform system,and the following results were obtained:the maximum age of UC patients included was 76 years old,the minimum age was 17 years old,and the average age was about 47.6 years old.Among them,58 males and 45 females showed no significant gender difference.Syndrome and activity analysis:the main syndromes of the patients were dampness-accumulation syndrome of spleen deficiency,followed by dampness-heat syndrome of large intestine,spleen-kidney deficiency syndrome of Yang deficiency of spleen and kidney,mixed syndrome of cold and heat,syndrome of heat toxicity,syndrome of liver-stagnation and spleen-deficiency,syndrome of Yin and blood deficiency,mainly in remission period.Analysis of pharmacokinesis of drug taste:director zhou used both cold and warm to treat this disease,and used 1621 times(30.25%)of warm drugs and 1556 times(29.04%)of cold drugs.The main flavor was bitter(32.40%),gan(31.10%)and xin(21.63%).The drug to spleen,stomach meridian is more common Among the 309 prescriptions,there were 97 traditional Chinese medicines.The top 10 herbs with the highest frequency of use were atractylodes,radix paeoniae,cocos,licorice root,coix seed,codonopsis codonopsis,bran Fried coix seed,ground elm,rhizoma coptidis and ginger charcoal.Based on the analysis of the medication law of association rules,it is concluded that the basic medication combination of director zhou zhenghua in the treatment of this disease is codonopsis,atractylodis,pachycocos,licorice root,white peony root,coix seed,bran Fried coix seed,rhizoma coptidis,radix rubediae,and ginger charcoal.Analysis of drug correlation degree and new prescription based on hierarchical cluster analysis:16 pairs of core combinations for director zhou's treatment of UC were obtained:(radix linderae,cortex magnoliae,(2)of semen raphani nutmeg,with sauce,rhizoma coptidis(3)semen plantaginis,big blood rattan,rhizoma atractylodis(4)semen plantaginis,licorice root,dried tangerine or orange peel(5)poria cocos,angelica sinensis,rhizoma atractylodis 6 big blood cane,sweet,dried ginger 7 Chinese bulbul,purslane,indigo naturalis was ze LAN,garden burnet,perrin pet-name ruby radix bupleuri,bran Fried semen coicis,magnolia bark attending radix scutellariae,white hyacinth bean,radix puerariae,rhizoma coptidis(11),semen plantaginis stachyurus,rhizoma atractylodis,dried tangerine or orange peel(12)plantain seed,big blood rattan,licorice,dangshen(13)angelica,radix aucklandiae,radix bupleuri,purple Perilla(14)malaytea scurfpea fruit,radix aucklandiae,dried ginger(15)qin,Chinese bulbul,cortex phellodendri,purslane(16)patchouli,perrin,big belly skin.Further New Zealand first 8:(1)medicine,magnolia bark,radish seed,radix bupleuri,bran Fried semen coicis(2)the nutmeg,with sauce,rhizoma coptidis,radix scutellariae,white hyacinth bean,puerarin(3)semen plantaginis,big blood rattan,rhizoma atractylodis,stachyurus,dried tangerine or orange peel(4)semen plantaginis,licorice root,dried tangerine or orange peel,large blood rattan,dangshen tenants-in-chief,poria cocos,angelica sinensis,rhizoma atractylodis,radix aucklandiae,radix bupleuri,purple Perilla 6 big blood cane,sweet,dried ginger,malaytea scurfpea fruit 7 Chinese bulbul,purslane,indigo naturalis,cortex fraxini,cortex phellodendri today ze LAN,garden burnet,perrin,patchouli,big belly skin.Analysis of the amount of common drugs and the taste of prescription drugs:the amount of commonly used drugs in zhou shi was 6g?30g,and the amount of prescription drugs was 14-20.Analysis of main syndromes:the main syndromes of spleen-deficiency and dampness-accumulation were lukewarm,and the main syndromes were sweet and bitter.The frequently used Chinese medicines are tuckahoe,atractylodes,codonopsis,licorice root,white peony root,bran sauteed coix seed,ginger charcoal,ground elm,Chinese yam,coix seed,cranioides,and bean paste The large intestine dampness-heat syndrome is mainly used in cold,with the most bitter,sweet and astringent taste.The frequently used Chinese medicines were rhizoma coptidis,rhizoma atractylodis,coix seed,white peony root,ground elm,radix puerariae root,beaten sauce,scutellaria,bran Fried coix seed and angelica.The spleen-kidney Yang deficiency syndrome is mainly used in terms of temperature,with a taste of sweet and bitter.The top 10 drugs with high frequency of use were atractylodes,poria cocos,licorice root,radix codonopsis,dried ginger,white peony root,psoraleae,nutmeg and coix seed.2 Research on the molecular mechanism of network pharmacology of zhou zhenghua's combination of basic drugs in the treatment of ulcerative colitisA total of 9 traditional Chinese medicines contained 81 chemical components.All the potential targets of the above components were collected,and 200 potential targets were obtained after sorting out,comparing and deweighting.After searching GeneCards and Digsee databases,a total of 2408 UC related genes were obtained.Compared with the aforementioned targets,137 common genes were obtained.The composition-target relationship was used to construct the basic drug-active component-target-disease network,and 48 active components were obtained.According to the protein interaction network of common targets,AKT1,CASP8,CDKN1 A,IL10 and other targets were found to be in the core position.GO further enrichment of functional analysis,KEGG pathway enrichment analysis shows that the drug combination could by participating in cytokines receptors,receptor ligand activity and cytokine activity,ubiquitin protein ligase combination,RNA polymerase ?transcription factor binding and other biological processes and PI3K Akt,IL-17,toll-like receptors signaling pathways play a role in the treatment of UCConclusions:1 Study on the medication law of zhou zhenghua in the treatment of ulcerative colitis based on data mining methodZhou zhenghua,director of the treatment of UC cold and temperature,mainly bitter,spicy,sweet drugs,mostly involving the spleen,stomach.The basic drugs are radix codonopsis,poria cocos,atractylodes,licorice root,rhizoma coptidis,ground elm,bean paste,white peony root,coix seed,bran Fried coix seed,ginger charcoal.From the perspective of the main syndromes such as dampness-accumulation syndrome of spleen deficiency,large intestine damp-heat syndrome,spleen-kidney Yang deficiency syndrome and so on,it is based on strengthening spleen clearing damp and regulating qi and blood.Evidence of large intestine damp and hot,add qingrejiedu,cool blood hemostasis products;The syndrome is the spleen and kidney Yang deficiency,add warming Yang qi,warm by hemostasis.The new recipe of nutmeg-beancurd-rhizoma coptidis-radix scutellariae-white hyacinth-radix puerariae-radix puerariae,paeturong-purslane-qingdei-qin PI-guan huang bai,can be used as the basis of large intestine dampness and heat syndrome and heat toxicity and heat heat syndrome respectivelyOn the characteristics of drug use,always start with the harmony of qi and blood,cold and warm,to spleen first,with clearing damp toxicity,cool blood hemostasis throughout.Overall syndrome differentiation,treatment by stages,the first phase of the activity to re-exorcise evil,do not forget to support;In the remission stage,the spleen and kidney were enriched,and the evil spirits were exorcised.It shows that zhou shi keeps the pathogenesis and USES the medicine flexibly when treating UC2 Research on the molecular mechanism of network pharmacology of zhou zhenghua's combination of basic drugs in the treatment of ulcerative colitisThe basic drug combination of zhou's treatment of UC contains a total of 48 effective ingredients for the treatment of UC,involving 137 targets,which may play a therapeutic role through the PI3K-AKT signaling pathway,IL-17 signaling pathway,Toll-like receptor signaling pathway and other biological processes such as cytokine receptor binding and cytokine activity.This study revealed the complex mechanism of treating UC by tutor's combination of basic drugs with UC through multi-target,multi-pathway and multi-pathway,and provided reference for subsequent studies. |
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