Inhibitory Effect Of Ginsenoside Rh2 On Proliferation Of Prostate Cancer Cell Line PC3 Induced By LPS

ObjectiveLipopolysaccharide LPS was used to observe the effect of TLR4 inflammatory pathway activation on the growth of prostate cancer(Prostate cancer,PC)cell line PC3,.By replicating the inflammatory model in vitro,the extract of Panax ginseng Rh2(ginsenoside Rh2,GRh2)was selected as the research drug to explore its effect on the proliferation of prostate cancer cell line PC3.The molecular mechanism of improving the treatment of prostate cancer was studied from the point of view of inflammation and androgen receptor AR.Methods1.The effect of the activation of TLR4 inflammatory pathway on the growth of PC3 cells: In vitro culture of prostate cancer cells PC3,the TLR4-specific activator lipopolysaccharide LPS intervention was given,and the proliferation and growth of the cells were observed in comparison with the control group(Control group);the secretion of IL-6 and TNF-1 in the supernatant of the supernatant was detected;At the same time,the expression of NF-kB p65,p-NF-kB p65,IkB-1 and p-IkB-1,and the expression of TLR4,AR,NF-kB p65,and the expression of TLR4,AR,NF-kB p65 were also examined.growth of PC3 cells.2.The effect of ginsenoside Rh2 on PC3 proliferation of prostate cancer cells induced by LPS:PC3 was cultured in vitro.The secretion of IL6 and TNF? in supernatant was detected.The expression of inflammatory related proteins NFkBp65,p NFkBp65,IkB? and p IkB? and mRNA expression of TLR4,AR and NFkBp65 were detected.And the inhibition of the proliferation of the prostate cancer cell PC3 is induced.Results1.Compared with the control group,the OD value of prostate cancer cell line PC3 in LPS group was up-regulated after 12 h,18 h and 24 h,and the proliferation trend was observed in the whole,in which the proliferation was more obvious at 18 h and 24 h(P < 0.01),and there was a significant difference between the two groups(P < 0.05).LPS had a time-dependent effect on the proliferation of PC3 cells.When treated with LPS for 24 h,the secretion of IL-6 and TNF-? in the supernatant increased significantly(P < 0.01),while the expression of NF-kB p65 and IkB-? did not change significantly(P < 0.01).After 24 h treatment,the mRNA expression of TLR4 and AR,NF-kB p65 in LPS group was significantly higher than that in Control group(P < 0.01).2.Compared with LPS group,OD value of Rh2 group decreased after PC3 treated with Rh2(P < 0.05),and there was a significant difference between the two groups(P < 0.05).Rh2 had a time-and concentration-dependent effect on the proliferation of PC3 cells treated with LPS.Compared with the LPS group,the secretion of IL-6 and TNF-? in the supernatant of the Rh2-60 LPS group decreased significantly(P < 0.01),and that of the Rh2-60 LPS group decreased even lower than that of the Rh2-60 group(P < 0.05),compared with the control group(P < 0.05).The difference was statistically significant.Rh2 processing for 24 hours,compared to the LPS group,The expression of NF-kB p65 and IkB-? did not change significantly(P < 0.05),but the protein phosphorylation level of Rh2-60 LPS group was lower than that of Rh2-60 group(P < 0.05),but the protein phosphorylation level of Rh2-60 LPS group was significantly lower than that of Rh2-60 group(P < 0.05,P < 0.05).After 24 h treatment,the mRNA transcription level of TLR4 and AR,NF-kB p65 in Rh2-60 group was significantly lower than that in LPS group(P < 0.01),and that in Rh2-60 LPS group was lower than that in Rh2-60 group(P < 0.05).),the difference was statistically significant.Conclusion1.The activation of TLR4 in 1.PC3 cells can increase the level of intracellular inflammation,which may be related to the activation of androgen receptor AR and cell proliferation.2.Ginsenosides Rh2 can inhibit the activation of TLR4 in PC3 cells and decrease the level of intracellular inflammation,which may help to down-regulate the expression of androgen receptor AR and inhibit the growth of PC3 cells.