Study On Fast Parallel Measurement Method For Three-dimensional Tracking Of High-throughput Microspheres Based On GPU

Three-dimensional position measurement of microspheres is an important part of single-molecule force spectrum technology.The numbers of individual measurements required for achieving a statistically reliable result can sometimes be proved daunting.Time-consuming and labor-intensive works have spawned the real-time tracking methods of large-scale single-molecule force spectrum technology.The work of this thesis is mainly based on the home-built digital holographic centrifugal microscope system,which could apply centrifugal force to hundreds of single molecules in parallel.In order to obtain high-throughput single-molecule stretching information in real time,we study the three-dimensional position measurement method of microspheres,and combine GPU acceleration technology to complete the high-precision and real-time parallel measurement of high-throughput microspheres.The main works can be summarized as follows:1.The background of and development status of microsphere three-dimensional position measurement at home and abroad are reviewed,then with a summary of the main application of microsphere three-dimensional position measurement in biological and physical research.Furthermore,we analyzed the technical barriers to be solved,and introduced the GPU acceleration technology to solve the microsphere tracking speed bottleneck.2.Combining with the self-built digital holographic centrifugal microscopy system,simulation of the microsphere imaging effect in the system is carried out,which provides a standard for the accuracy evaluation of subsequent algorithm.3.The temporal and spatial resolution of various common positioning algorithms are compared and analyzed.Combined with the imaging characteristics of the system,a new method for the system is proposed.Utilizing the GPU acceleration technology,main parameters affecting the software designed for CPU+GPU hybrid programming,including speedup and accuracy,were proposed by simulation and practical experiments.The spatial resolution of the algorithm can reach 1nm.Together,the software allows us to simultaneously track hundreds of microspheres in centrifugal microscopy in real-time(500 beads at 70Hz).4.The force spectrum stretching measurement was performed on the double-stranded dsDNA,and the force spectroscopy curves were analyzed to verify the availability of the hardware system and the effectiveness of the software system in terms of accuracy and speed.