Chromatographic Workstation Design And Apectral Analysis Algorithm Research

Chromatographic is a method to separate components by using the different distribution effects of different components in the fixed phase and mobile phase.It has the characteristics of high separation efficiency,high selectivity,high sensitivity and fast analysis speed,so it has been widely used and developed rapidly.Based on the advantages and disadvantages of current chromatographic software at home and abroad,a network server-based supercritical fluid chromatographic workstation is designed and developed in this paper.The development environment and related application technology of chromatographic workstation are introduced in this paper.B/S structure is adopted by software,and Dajango in Python is used as server application framework.HTML5 and JavaScript technology is used to design the front-end web page,and Ajax is used for the interaction between the front and rear ends to realize the dynamic update of the page.The chromatographic instrument is communicated with the server via RS232 asynchronous serial port.Data storage adopts the method of combining static storage and cache to improve the efficiency of data access.In order to improve the response speed and stability of the software,asynchronous queue task processing process are used in server.The chromatographic workstation software has realized the main functions of data storage,user management,equipment monitoring and chromatographic analysis,and has carried out system test.Spectral data processing is the core part of chromatographic workstation.The first is data preprocessing.In order to reduce the data pressure transmitted by the server,Wavelet transform is used to compress and decompress the data,and the signal can be recovered well before and after compression.S-G filter and wavelet transform are combined to de-noise and smooth the spectrum effectively.The second derivative method is used to identify the spectrum peaks,which can identify different chromatographic peaks accurately.The N-M fitting method based on EMG model is used to separate the overlapping peaks,and achieve a good separation effect.Finally,the methods of computing the characteristic value of chromatographic peak and qualitative and quantitative calculation are introduced.