Research On The Viability Detection Technology Of Mesenchymal Stem Cells Based On Light-induced Delayed Luminescence

Human Umbilical Cord Mesenchymal Stem Cells?h UC-MSCs?have the advantages of strong self-renewal capacity,multi-directional differentiation potential,easy access to materials,no ethical issues involved,low immunogenicity and so on.They have a broad application prospect in drug research and development,clinical application and other fields.Whether in drug research or clinical application,cell viability is a key factor that needs close attention.Although there are many biochemical methods that can detect cell viability,most of these methods are usually invasive and may cause irreversible damage to cells,and it is difficult to meet the quality control requirements of stem cell production.As an extension of biological spontaneous illumination,light-induced delayed luminescence contains abundant information of life activities,which can reflect the changes of physiological and pathological state of organisms sensitively,and is expected to become a new method of label-free and non-invasive cell viability detection.In this paper,based on the advantages of optical label-free and non-invasive,we use light-induced delayed luminescence technology to characterize the changes of mesenchymal stem cell viability,in order to explore a non-destructive method to detect cell viability.Major achievements of this paper include three parts:Firstly,the basic characteristics of delayed luminescence of h UC-MSCs are studied.Specifically,it includes:cell spacing,number of living cells,cell viability,light intensity,light time and wavelength of irradiation.The research results show that:1.The delayed luminescence of h UC-MSCs is mainly induced by ultraviolet light;2.Three important conditions that form the long-life delayed luminescence of h UC-MSCs are the participation of living cells,cell precipitation and a sufficient number of cells;3.Under the same light-induced condition,the time of detecting the delayed luminescence signal is related to cell viability,the higher the viability,the faster the delayed luminescence signal is detected;4.Cell viability is a n important factor affecting the delayed luminescence of h UC-MSCs,under the same light condition and the same number of cells,the higher the cell viability,the higher the delayed luminescence intensity and the slower the delayed luminescence decay rate.The results revealed that the delayed luminescence of h UC-MSCs is closely related to the life activities of cells.Secondly,based on the analysis of the basic characteristics of delayed luminescence of h UC-MSCs and the discussion of the delayed luminesce nce mechanism,a mathematical model,the theoretical model of biophoton coherence,is proposed to analyze the delayed luminescence process of h UC-MSCs.It is verified that the fitting degree of the model to the experimental data is higher than that of the traditional hyperbolic decay function model,and the physical meaning of each parameter in the model is clear,which can be used to analyze the decay dynamic characteristics of h UC-MSCs delayed luminescence.Finally,the theoretical model of biophoton cohe rence is used to fit the labeled high and low viability delayed luminescence curve of h UC-MSCs,through statistical analysis,it is found that the two parametersN_S/N₀??-G₀ can be used to characterize the change of the viability of h UC-MSCs,which lays a foundation for the development of non-destructive testing method of the activity of h UC-MSCs based on delayed luminescence technology.