Study On The Electrochemical Sensor Of Copper-adenine Coordination Polymer And Carbon Material Prepared From It

Metal-organic Frameworks(MOFs)possess large specific surface area,the rich spatial structure,and mutil-functionality.They have become a research hotspot in recent years which attracted scientific researchers' widespread attention.Metal-biomolecule frameworks(MBioFs),which are defined as MOFs constructed from at least one biomolecule which serves as an organic linker building blocks,is a kind of special MOFs material.Some biomolecules not only have the pore structure and large specific surface area of MOFs,but also have interesting recognition.Even some possess unique biological,and emerge as very promising materials for luminescence,gas adsorption and catalysis,which have received tremendous attention in coordination chemistry and materials chemistry.Adenine,a biomolecule,is a base that compose human beings genetic materials DNA.The synthesis of this kind of MBioFs comprised by adenine molecules as organic ligand has been studied very since the 1960s,and the study of luminescence properties and pore and crystals about this kind MBioFs have been reported.However,the electrochemical study of the MBioFs frameworks is still rare.In this thesis,we prepared a novel bifunctional electrochemical biosensor based on the MBioFs.At the same time,we synthesized a kind of CNx and prepared an electrochemical sensor.The main contents are as follows:1.The preparation and characterization of Cu-Ade and the bifunctional electrochemical biosensor based on Cu-AdeCPE.The Cu-Ade was prepared by copper salt,and biomolecule adenine.Copper ion own the empty d orbital,variable valence state and unsaturated metal sites.We studied the effect of surfactant,the result shows that adding the surfactant PVP,the size of the product particles changed.Cu-Ade was synthesized by stirring method and characterized by various techniques,including XRD,and TEM.The XRD result indicates that Cu-Ade is the target material.TEM images show that PVP reduced greatly the size of the obtained Cu-Ade and the size of particles changed to micro-nano when adding the surfactant.Cu-AdeCPE was used to construct hydrogen peroxide and phenformin electrochemical sensors.Cu-AdeCPE showed novel bifunctional electroanalysis ability toward phenformin and the reduction of H2O2 due to adenine and Cu,respectively.The sensor show good electroanalysis performance to phenformin with a linear of 5.0×10-6 to 5.5×10-4 mol·L-1,a detection of 4.0×104 M and a sensitivity of 856 mA mol-1 L cm-2.-0.2V was chosen to obtain good performance for reductive determination of H2O2,and the linear range was 5.0×10-5 to 2.0×10-3 mol·L-1,the detection limit was 1.0×10-5mol·L-1 and the sensitivity was 94.0 mA mol·L-1 cm-2.Cu-AdeCPE also showed high selectivity,steadiness and reproducibility.The bifunctional electroananlysis ability and the excellent performance of the MBioFs frameworks imply that they are used as promising candidates in electrochemical sensing.2.The VB2(vitamins B2)electrochemical biosensors based on CNx/GCE.CNx were prepared from Cu-Ade.CNx was synthesized by calcine and characterized by XRD,IR,and TEM.The XRD result indicates that the prepared CNx has the peak at 250 corresponding to carbon.TEM images shows that the synthesized sample is irregular layer structures particles.The width of the sample is 500nm and the length of it is 3.0-5.0?n.Then we made CNx glass carbon electrode to study electro-catalytic reduction of VB2.The developed VB2 electrochemical biosensor showed a high sensitivity of 399 mA mol-1L Cm-2,with a linear of 6.0×10-6 to 3.5×10-4 mol·L-1,a detection of 5.4×10-6 mol·L-1,which demonstrates that CNx has a great potential in the application of electrochemical biosensors.