Preparation Of Organic Macromolecule Functionalized Nanomaterials And Its Application In Sensing Platforms

Fluorescent and electrochemical sensors have become the research hot in the modern analysis method.Compared with traditional method,the fluorescent and electrochemical methods have great advantages,such as simple operation,low cost,few sample consumption,good selectivity,high sensitivity.Therefore,the sensors have been widely applied in the field of environmental protection,food monitoring,disease diagnosis,biomedicine.The nanocomposites of SCX6@RGO and PtPd-CP5@RGO were prepared by using the SCX6,CP5,RGO and PtPd material.Graphene nanomaterials have been demonstrated to possess unique electronic,chemical and structural properties.SCX6 and CP5 have been demonstrated to possess the good supramolecular recognition capability.The PtPd nanoalloy has been demonstrated to possess the high conductivity and catalytic activity.Furthermore,Au nanoclusters was synthesized in aqueous solution at 37? by using ovalbumin as stabilizer.The sensors of resveratrol,sunset yellow,bisphenol A,and CN" have been established by using the above nanocomposites.The main research contents of this paper are as follows:(1)A sensitive and selective fluorescent approach for resveratrol detection based on a competitive host-guest interaction between SCX6 and signal probe/target molecules using SCX6@RGO as a receptor was developed.Due to the outstanding quenching performance of the graphene and the excellent host-guest recognition of SCX6,the fluorescence method for the determination of resveratrol shows high sensitivity and good selectivity.The fluorescence analysis of the linear ranges of the sensor(SCX6@RGO � RhB)was 2.0-20.0 ?M and 20.0-40.0 ?M,with the detection limit of 0.47 p.M.The sensor(SCX6@RGO �R123)was 2.0-12.0 ?M and 12.0-20.0 ?M,with the detection limit of 0.56 ?M.(2)The CP5@RGO nanocomposite was prepared using grapheme(GO)and cationic pillar[5]arene(CP5)by a simple wet-chemical route.The Pt4+ and Pd2+precursor were reduced by NaBH4 and suppored on the CP5@RGO to form the PtPd-CP5@RGO nanocomposite.The electrochemical behavior and performance of BPA were studied.Due to the high catalytic activity of PtPd nanoalloy and the excellent supramolecular recognition capability,The electrochemical sensor show a wide linear ranges from 0.0 to 0.5 ?M,0.5 to 1000.0 ?M,with the detection limit of 0.003 ?M.(3)A simple and high sensitive fluorescence approach was established for the determination of SY in aqueous solutions using cucurbit[7]uril-luteolin/EGCG as fluorescence probe and SY as analyte.The competitive interaction between luteolin/EGCG and SY in the cucurbit[7]uril cavity was investigated by spectrofluorimetry and UV spectrometry.The inclusion complex of cucurbit[7]uril and SY was verified by molecular modeling.The linear ranges of fluorescent method based on the luteolin � CB[7]probe is 0.5?10.0 ?M,and 10.0?50.0 ?M,with a low detection limit of 0.12?M.The linear ranges of fluorescent method based on the EGCG � CB[7]probe is 2.0?30.0 ?M,and 30.0?50.0 M,with a detection limit of 0.45 ?M.(4)Ovalbumin stablized gold nanoclusters was synthesized under mild conditions.The blue and red luminescence are observed from Au nanoclusters by using different pH conditions.Furthermore,Au nanoclusters was applied to detect CN-in aqueous solution based on the fluorescence quenching effect.The linear ranges of this method is 0.5 to 10.0 ?M,and 10.0 to 400.0 ?M,with a low detection limit of 0.13 ?M.