Research On Integration Of Tumor Marker Detection And Diagnosis And Treatment Based On Nanomaterials

The detection and treatment of the tumor,a big threat to human health,has become a hot spot in the related field.This paper firstly synthesized the MnO₂nanaosheet,and then based on the MnO₂ nanaosheet design a nanoprobe with target identification.Combined with RCA circle amplification strategy,achieved the sensitive detection of intracellular GSH and tumor cell imaging of fluorescence/MRI dual-mode amplification system;Secondly,using DNA self-assembly and nucleic acid nanotechnology,combined with RCT circular amplification strategy,synthetic RNA nanohydrogel was constructed?And then the diagnosis and treatment integration system for compound drug delivery and targeted therapy was constructed.The main study contents are as follows:Here,we report a novel magnetic resonance imaging?MRI?/fluorescence bimodal amplification platform for the detection of glutathione?GSH?on the basis of redoxable manganese dioxide?MnO₂?nanaosheets,which can be easily applied as a DNA nanocarrier,fluorescence quencher,and intracellular MRI contrast agent activated by GSH.The binding of aptamers that absorbed on the MnO₂ nanaosheets to their target canfacilitating the endocytosis of target nanoprobes.Once endocytosed achieved,cellular GSH can react with the MnO₂ nanaosheets,resulting in the disintegration of nanaosheets to generate plenty of Mn²⁺ions for MRI and releases the primers which were adsorbed on the MnO₂ nanaosheets.Then the RCA reaction was initiated to amplify the fluorescence signal of incelluar GSH.Therefore,this MnO₂nanoprobe shows excellent selectivity,a potential detection platform for analyzing the glutathione level in cells was suggested.For the conventional cancer therapeutic and the anticancer gents that have some challenges in targeting and systemic delivery.Here we showed a synergism of gene,chemotherapy and phototherapy delivered through the RNA nanohydrogels?RNA NHs?.MicroRNA?miRNA?,as a gene expression master regulators,was a promising class of cancer therapy.In our design,the RNA NHs as a result of rolling circle transcription?RCT?contain polymeric three microRNA?let 7a,miR 34a,miR 145?hairpins within the same RNA nanohydrogel?three-in-one RNA NHs?.Doxorubicin?DOX?which is a chemotherapy drug,and 5,10,15,20-tetrakis?1-methylpyridinium-4-yl?porphyrin?TMPyP₄?,a general photosensitizer,were effectively embedded into the RNA NHs.The multifunctional RNA NHs were delivered to target cells efficiently and formed a synergism combined gene therapy,chemotherapy and phototherapy under the light irradiation.Therefore,RNA NHs,a drug release nanoplatform,have great potentials for multifunctional synergistic cancer treatment.