The Synthesis And Application Of GSH&pH Dual-control Drug Delivery Nano-silica Spheres And The Research And Application Based On BODIPY Fluorescent Probe

This paper can be divided into two parts:In order to improve the control efficiency of traditional single-condition controlled drug delivery system,we use inorganic mesoporous silicon nanomaterial?MCM-41?as the basic material to achieve pH and GSH dual controlled drug delivery system through surface modification;Based on the strong UV absorption,fluorescence emission and sorghum asset rate of BODIPY dye,we designed,modified and synthesized two organic small molecule fluorescent probes.The fluorescent properties of the probe,the recognition performance,and the test results in samples were systematically studied.1.The drug delivery system has received great attention in medical research and development and biological applications,and various new transmission systems have been developed.In this paper,we use imine group as the pH control trigger,while the disulfide bond with terminal alkynyl as the GSH control component.These functional groups are respectively modified on the surface of the mesoporous material in a certain order.After the drug loading,the capping effect of the double-controlled cyclodextrin was achieved by the recognition of the host-guest of amantadine and?-cyclodextrin and the copper-catalyzed click reaction.The successful preparation of dual-controlled nanospheres was verified by a series of characterizations.In the release test,the?-cyclodextrin failed to leave the guest when the normal conditions or single conditions were satisfied,because it played a good role as a"cap".When both conditions are satisfied,both the imine bond and the S-S bond can be rapidly broken,resulting in the departure of?-cyclodextrin and the release of DOX.2.A small molecule fluorescent probe for lysine detection was successfully modified the aldehyde group at the 3 position of BODIPY by the Vilsmeier-Haack reaction.Through a series of tests,we found that the probe can be well tested for amino acids in the system?DMSO:H₂O=9:1,V/V?with an optimal detection concentration of 20?M.The changes in UV and fluorescence intensity during the test were very obvious and had good sensitivity.And it exhibits excellent selectivity and anti-interference in more than ten common amino acids.3.The tetramethyly-BODIPY derivative containing an azobenzene structure is obtained by diazotization of tetramethyl BODIPY as a fluorophore.The fluorescence of BODIPY was quenched due to the strong electron withdrawing action of the azobenzene structure.At the same time,due to the sensitivity of the azobenzene structure to hypochlorite,the azo structure of the probe can be rapidly destroyed by hypochlorite and the fluorescence is restored.Through a series of conditions screening,we found that the probe can quickly detect hypochlorite in the test system?EtOH:H₂O=1:1,V/V?,the reaction finished in less than one minute,the optimal detection concentration is 5?M,detection limit is 70 nM.In the reaction to different RNS and ROS,only hypochlorite can react with the probe.The fluorescence of the system obviously changed after the reaction.The system had no fluorescence before the addition of hypochlorous acid while the fluorescence intensity increased rapidly after adding hypochlorous acid,which was bright and strong green fluorescence.A series of test results show that the probe is a excellent probe for hypochlorous acid detection.