The Functional Fluorescent Probe Is Used For The Detection Of Nitrofuran Drugs And Selenocysteine

High-performance liquid chromatography(HPLC)method has been widely used in food safety,environmental monitoring,and medical fields.The development of chemical derivatization methods can greatly improve the detection sensitivity.However,due to the low content of some tested substances and the complexity of their components,the application of HPLC has been limited.The development of fluorescently labeled reagents has made it possible to use HPLC methods for the detection of trace components.Therefore,it is of great research significance to develop a novel type of fluorescent labeling reagent and combine with high performance liquid chromatography for the detection of trace substances.Nitrofuran drugs are restricted for their carcinogenicity and teratogenicity.Thus,it is necessary to detect its abuse in food.The types and concentrations of some endogenous active substances(such as small molecules and ions)in living organisms are different,and their physiological functions are different.A detailed understanding of the production,distribution and physiological functions of related active substances is of great significance for studying ecological balance,physiological and pathological processes.Therefore,real-time dynamic monitoring of these active substances is necessary,and the development of fluorescence imaging just meets such needs.In addition,the development of small-molecule fluorescent probe technology provides certain possibilities for the early diagnosis of diseases.This article mainly exploits two derivatization reagents to quantitatively study nitrofuran drugs in food through microwave-assisted derivatization accompanied by high-performance liquid fluorescence detection technology.In addition,in order to study the physiological and pathological effects of selenocysteine(Sec),we designed and synthesized a ratio of near-infrared fluorescent probes and used them in thyroid disease.The changes of Sec content were detected in cell and thyroid mouse models.The specific work is as follows:Chapter one: The application of fluorescent labeling reagents in high performance liquid chromatography methods and the development and application of small molecule fluorescent probes were briefly introduced.Finally,the general situation of nitrofuran metabolites and their quantitative detection methods were also introduced.Chapter two: Using 7-(diethylamino)coumarin-3-carbaldehyde as derivatization reagent,the four nitrofuran metabolites were derivatized and analyzed by high performance liquid chromatography-fluorescence detection(HPLC-FLD).This method has been successfully applied to the quantitative detection of four nitrofuran metabolites in food.Chapter three: A fluorescent derivatization reagent,4-(acridone-10-yl)benzaldehyde,was successfully designed and synthesized,and applied to the detection of nitrofuran metabolites in shrimp samples by HPLC-FLD method.With the aid of microwave-assisted technology and response surface curve method,the derivatization conditions were optimized,which greatly reduced the reaction time.Under the optimized conditions,the quantitative detection of four nitrofuran metabolites in shrimp samples was successfully achieved.Chapter four: A small molecule fluorescent probe for detecting Sec content in cells and models of thyroid diseases was designed and synthesized.At the same time,a hydrogen peroxide probe was applied to measure the changes in hydrogen peroxide content in thyroid disease cells and mice.In addition,the anti-inflammatory and antioxidant effects of Sec in thyroid disease models were evaluated.