Ion Transmission Control Of Rigid-flex Block Copolymer Nanochannels And Preparation Of Peptide Solid-phase Synthesis Carriers

Bio-inspired smart asymmetric nanochannel membrane have received wide spread attention.Compared with traditional biological systems,synthetic materials have better stability and durability.Among these materials,block copolymers can self-assemble into ordered nanostructures“from bottom to top”.The molecular structure can be used to achieve the coordinated regulation of the inner surface chemical structure of the channel and the nanostructure of the channel to produce hierarchical ordered,high-porosity,controllable and functional intelligent asymmetric nano ion-channel membrane can make up for the defects of traditional etching and other methods.In this dissertation,an ionic block copolymer with hierarchical ordered structure is designed and synthesized,and its self-assembly structure and internal surface groups of the channel are studied.It was further compounded with porous polymeric nanochannels to obtain intelligent asymmetric nanochannel membrane with high strength and low internal resistance.The ion transmission and osmotic energy generation properties of the composite membrane were studied.In addition,we have adopted a new idea and proposed a feasible route for solid-phase peptide synthesis and in vivo screening,which provides a new path for the preparation of peptides with tumor targeting capabilities.The main results of research and experimental work done in this paper are as follows:?1?The polystyrene-poly-?-3-chloropropyl-L-glutamic acid block copolymer?PS-NH-PCPLG?was successfully prepared to construct a hierarchical ordered assembly structure.Subsequently,it was modified to prepare the ionic block copolymer PS-NH-PPLG-g-BIB.The highly charged imidazolium functional group at the end of the chain aims to improve the ion selectivity in the channel.The hierarchical structure constructed with polyglutamic acid derivatives as the main chain as an ion transmission channel can effectively improve the channel order and increase the transmission rate.The helical rigid secondary structure of the peptide molecule also avoids entanglement of the molecular chains and ensures that the functional groups are exposed.?2?The ionic block copolymer PS-NH-PPLG-g-BIB and the porous polymeric membranewere compounded to prepare a thin film with asymmetric nanochannels.with asymmetric surface charges and nanostructures can eliminate concentration polarization and regulate unidirectional ion migration of the channel.Finally,the output power density of salinity gradient power generation of 4.62 W/m² can be realized,revealing the huge potential of hierarchical ordered structures in salt differential energy collection.?3?Fe₃O₄@PS microspheres were prepared,and?4-?hydroxymethyl?phenoxymethyl?styrene was modified on its surface as Linker.The carrier was used for solid-phase peptide synthesis,which verified its feasibility as a solid-phase carrier and provided a new idea for the in vivo screening of peptides.