Research On Phase Recovery And Multi-mode Imaging Based On Light Intensity Transmission Equation

As one of the greatest inventions of the twentieth century,microscopes have now penetrated into various fields of scientific research.In order to avoid dyeing of transparent samples,researchers have proposed a variety of methods that can be used to achieve unmarked phase measurement and phase retrieval.Because of its advantages of non-interference,non-iteration and unwrapping,transport of intensity equation has gained wide attention and research from researchers both at home and abroad.The method does not require complex interference devices,and avoids the uncertainty of iterative algorithm.And it can achieve quantitative phase imaging on the traditional bright field microscope by simple transformation.Aiming at these characteristics of TIE imaging,this paper focuses on researching and putting forward two kinds of phase imaging system based on transport of intensity equation:namely multi-mode imaging system and Optical diffraction tomography system.The multi-model imaging system combines the traditional bright field microscope with the computing imaging.The image stack of different focal planes is obtained by the electrically controlled zoom lens module.And the quantitative phase images,Zernike phase contrast images and DIC images are obtained through calculation.The results of living cell imaging show that the system can be effectively applied to the biomedical research of cell morphology.Optical diffraction tomography system which transforms the traditional bright field microscope by using the programmable LED array as the illuminantion light source obtains the complex amplitude of the forward scattered light field by utilizing the TIE phase retrieval method.Finally,the three-dimensional refractive index distribution of the object is obtained by using the optical diffraction tomography algorithm and the non-negative constraint iteration.The system is different from the traditional method based on digital holography,and the non-interfering three-dimensional phase tomography is realized.The experimental results of two phase imaging systems based on TIE imaging proposed in this paper show that TIE quantitative phase imaging technology has potential application prospects in life sciences,biomedicine and pathological sectioning.