A Method For Detecting The Components Of Matsutake Using GC-MS Was Established

Tricholoma matsutake is a large edible medicinal fungus,has various special values of anti-tumor,enhance immunity.So get more and more attention from all over the wor d.In view of detection methods are not perfect and difficile true problems at present,this paper selects 14 kinds of samples as research material,the best method for detection of volatile and non-volatile components were established by GC-MS and carries on the methodological study and application in order to achieve the purpose of quality evaluation and good classification and distinction of them.Results are as follows:The optimum conditions of GC-MS to determine the volatile components of T.matsutake is headspace injection with split ratio 2:1,the initial column temperature was 40? keeping 5min,then heating at 4'C per minute to 120 C keeping 2min.After that heating at 5 C per minute to 280 C,vial equilibration temperature was 140 C for 20min.The optimum conditions of GC-MS to determine the non-volatile components of T.matsutake is inlet temperature 300? with the column flow rate at 1.2mL/min,the initial column temperature was 80?,keeping 2min,heating at 8? per minute to 165?and keeping 2min,and then heating at 1? per minute to 180 C keeping 2min,and then heating at 20'C per minute to 220 C keeping 2min,finally heating at 5? per minute to 300 C keeping 5min.Data required by whole scanning with acquisition range 70-700 m/z.The two established methods have been measured by methodology,The precision,repeatability and stability meets the requirements of fingerprinting.Identified 20 common peaks can be used as one of indexes of T.matsutake volatile components,and 23 common peaks as one of the indicators of T.matsutake non volatile components.Manual analysis of volatile components of T.matsutake had identified a total of 293 substances,with alcohols,aldehydes,ketones,olefin and nitrogen heterocyclic are major compounds;and of non-volatile compounds of T.matsutake had identified 129 substances,with sugars,alcohols,acids and esters are major compounds.The differences of 14 kinds of T.matsutakes with different geographical origin,ways of storage and picked years are well classified and distinguish by similarity analysis and cluster analysis.Outlier degree of samples are significantly proved that those T.Matsutake are counterfeit which from Shandong linyi,Guangzhou shenzhen and Zhejiang lishui.The results provide a certain basis for T.matsutake authenticity identification.Comprehensive analysis,the established detection methods of GC-MS with high precision,good repeatability,and can qualitative analysis to T.matsutake.Able to the distinction difference quality between different sources of T.matsutake samples and identification quality of T.matsutake.