Analysis Of The Chemical Constituents And Body Constituents Of Cistanche Cistanche

Cistanches Herba is a famous traditional Chinese medicine with complex chemical composition.Traditional analytical methods are usually focused on phenylethanoid glycosides and it is difficult to achieve comprehensive quality control for the herba.Cistanches Herba has extremely high medicinal value for its treatment of anti-dementia,anti-parkinsonism,protecting cerebral ischemia and improving learning and memory.Cistanche glycosides are the main active ingredients which widely used in clinical to prevent and treat vascular dementia.However,the effective substances and action mechanism of Cistanche Herba in vivo have not been clearly defined,which seriously restricts its clinical application and further development.In order to clarify the chemical components and improve the quality control system of Cistanches Herba,the chemical components of Cistanches Herba were qualitatively and quantitatively characterized.1.Aiming to analyze the chemical profile of Cistanche herba(CH),in-lab database was established and fragmentation pathways of primary chemical types were summarized on the basis of literature datas.Structural identification was efficiently and accurately identified for the main constituents via HPLC-IT-TOF-MS,a total of 45 compounds were definitely assigned in negative mode.2.The contents of 23 hydrophilic and hydrophobic substances which spanned wide polarity(-3.326?cLogP?1.421)were quantified in 20 batches of Cistanche herba using serially coupled reversephase-hydrophilic interaction chromatography-tandem mass spectrometry(RPLC-HILIC-MS/MS),including three organic acids(i.e.nicotinic acid,succinic acid,and ferulic acid),four nucleosides(i.e.uridine,adenosine,inosine,guanosine),two carbohydrates(i.e.mannitol and surcose),one amino acid(i.e.phenylalanine),one benzyl-glucoside(i.e.cistanoside F),two lignans(i.e.alaschanioside A and pinoresinol-?-D-glucopyranoside),seven phenylethanoid glycosides(i.e.salidroside,echinacoside,cistanoside A,tubuloside A,acteoside,isoacteoside,and 2'-acetylacteoside)and three iridoids(i.e.gluroside,6-deoxycatalpol and 8-epi-loganic acid).On the other side,inferior parameters rather than the optimal ones were applied for those abundantingredients to advance the upper limits of quantitation,such as echinacoside(1.35-387.50 mg/g),mannitol(13.45-110.00 mg/g),and acteoside(0.75-85.90 mg/g),in mass spectrometer domain.Simultaneous quantification of the analytes that spanned wide content range(more than 5 orders of magnitude)was achieved.RPLC-HILIC-MS/MS was configured for simultaneous determination of components with wide polarity andcontent ranges in CH,which provided a basis for quality standard ascension of CH and contributed to comprehensively understanding the metabolome of plants.To elucidate the effective substances and action mechanism,the chemical composition of Cistanche Herba in vivo was qualitatively and quantitatively characterized.1.To elucidate effective material basis and metabolic pathways of CH in vivo,in-lab database and fragmentation pathways was built based on literature datas,45 compounds assigned in CH and extensive phase I and phase II metabolic pathways.Metabolites in plasma,urine and feces of rats were systematically characterized after oral administration of CH water extract by HPLC-IT-TOF-MS.A total of 82 prototypes and metabolites were tentatively identified,including 35 in plasma,36 in urine and 60 in feces,respectively.The results indicated that the main compounds of CH were easily hydrolyzed and metabolized into degradation products(i.e.caffeic acid,hydroxytyrosol and phenolic acid derivatives).Deglycosylation,sulfation,methylation,acetylation,hydroxylation,hydrolysis,oxidation,reduction and glucuronidation were considered as the primary metabolic pathways for the main constituents of CH.Moreover,sulfation,hydroxylation and degradation mainly occurred in plasma,sulfation and glucuronidation almost occurred in urine while most compounds found in feces were the prototypes.2.To illustrate pharmacokinetic characteristics of constituents migrating in blood,pharmacokinetic profiles of 19 compounds including endogenous components,drug-derived components were researched in rat after oral administrationof CH using RPLC-HILIC—MS/MS,and concentration-time curves and pharmacokinetic parameters were obtained.This research preliminarily elucidated the chemical constituents,metabolic pathways in vivo and pharmacokinetics characteristics of components commigrating in blood of CH,which provided reference for CH efficacy material basis research,action mechanism and clinical use.