| Objective:In the clinical practice of bone injury and tendon injury,more and more topical drugs are used,and the transdermal absorption of topical drugs is the focus of current research.The traditional microneedle in the transdermal has great damage to the skin and is prone to bleeding.In addition,the contact with topical drugs is easy to cause infection.Latest research new nano micro needle use and cosmetic medicine,transdermal research has some basic experiment support,the main purpose of this experiment is to analyze the new and the traditional micro nanometer micro needle and promote through technology of diclofenac sodium gel in vitro comparative study the effect of absorption,for fractures muscle injury transdermal study found that the new technology to promote and provide theoretical basis.Experimental materials and methods:(1)in vitro transdermal experiments on animals:this experiment was divided into A blank control group(group A),A new nano micro group(group B),and A traditional micro needle group(group C),with 3SD rats in each group.(1)animal skin treatment:the grouped healthy SD rats were removed with a shaving knife to remove the abdominal hair,cervical dislocation and death,immediately take abdominal skin,size 2*3cm~2,remove subcutaneous adipose tissue and mucus tissue,rinse with normal saline.Filter paper after blot moisture,spread in the aluminum foil,wrapped,at 20?,thawed use within a week.(2)the grouping in vitro transdermal experiments:each group of the TK-12 type a Franz diffusion test device,each team will take the animal skin thawing,group B and group C,respectively,using nanometer micro needle and the traditional micro on mouse skin,keep the needle body and skin surface vertical,along the longitudinal axis pressure needle array(1,N)and continue to function after 2 min to remove,wash.The skin of blank mice was not treated.The skin was fixed between the administration chamber and the receiving chamber in the diffusion pool,with the medial side of the mouse skin facing the receiving chamber and the cuticle facing the administration chamber.The effective contact area of the two Chambers was 3.14cm 2,the volume of the dosing chamber was 6.5ml,and the volume of the receiving chamber was15mL.The receiving solution was 0.9%sodium chloride solution,and the feeding chamber was 2mg sodium bisphenolate gel+2ml0.9%normal saline.Water bath temperature is 32?,speed 600 r/min.Sample 1 mL of 1,2,4,6,8,10 and 12respectively in the receiving tank,and replenish the same amount of receiving liquid at the same temperature.After derivatization,the sample was filtered by 0.22 m microporous membrane,and the concentration was determined by UPLC to calculate its cumulative permeability and permeability coefficient.The experimental data were expressed as x SD,and the experimental data were statistically analyzed by one-way anova,and the differences between the two groups were compared by t test.When P<0.05,there were significant differences between the two groups.(2)histological experiment:the skin above completed in vitro transdermal experiment was fixed in10%formaldehyde solution(formalin)and embedded in paraffin.Hematoxylin-eosin(HE)staining was performed to prepare skin longitudinal section pathological sections.The skin structure was observed under an inverted microscope and photographed.Results:1.The 12h cumulative permeability of group A was 116.93±1.65ug/cm~2,and the diffusion rate was 9.076.The cumulative permeability of group B was 160.9±2.19ug/cm~2for 12h,and the diffusion rate was 12.164.The cumulative permeability of group C for 12h was 199.56±2.63ug/cm2,and the diffusion rate was15.576.Compared with group B,P<0.05,there was A significant difference between the two groups,A<B.Compared with group C,P<0.05,there was A significant difference between the two groups,A<C.Compared with group C,P<0.05,there was a significant difference between the two groups,B<C.2.Changes in the skin structure of SD rats in group A,B and C:no significant changes were observed in the skin structure of SD rats in group A,and large pinholes were observed in the dermis of group C,which caused severe damage to the subcutaneous tissue.In group B,there were fine pinholes in the dermis,and the subcutaneous tissue was less damaged.Conclusion:(1)traditional microneedle and new nano-microneedle transdermal osmosis promoting technology have obvious osmotic promoting effect.(2)the penetration promotion effect of traditional micro needles is better than that of new nano micro needles.(3)compared with traditional microacupuncture,the new nano-microneedle has less damage to the skin structure,small pinhole,no pain,no bleeding,lower risk of infection,convenient to carry,reversible recovery,and more suitable for clinical exploration of new technology for transdermal drug delivery and further research,laying a foundation. |
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