| Purpose:The purpose of this study was to establish C57BL/6 mice model of cholesterol gallstone induced by high fat diet,based on the observation of piperine on high fat diet induced C57BL/6 mice intestinal absorption of cholesterol gallstone formation,transport and other effectsMaterial and method:1.Model establishmentMale C57BL/6 mice,6-8 weeks old,weighing 19-21 g;control group?10 rats?,feeding normal diet,model group?10 rats?,high fat diet?containing 2%cholesterol,0.5%bile acids and 15%fat?,for 4 weeks.2.Experimental grouping and AdministrationThe mice were randomly divided into five groups:control group,model group,20 mg·kg-1 piperine dosage group and piperine 40 mg·kg-1 dose group and piperine 60 mg·kg-1dose group,10 rats in each group.3.Experimental methodThe content of total cholesterol,triglycerides,high-density lipoprotein cholesterol,low density lipoprotein cholesterol,alanine aminotransferase and total bile acid in serum was detected by automatic biochemical analyzer;Western blot was used to detect the expression of NPC1L1 and SB-BI protein in intestinal jejunal epithelial cells of mice.Results:1.Model establishmentAfter observation,the mice in the blank control group had no stone formation,and the mice in the model group all formed stones,and the stone formation rate was 100%.2.Effect of piperine on serum total cholesterol in cholesterol gallstone miceCompared with the blank control group?2.4740+0.2884?,the total cholesterol content in the model group?3.3420+0.2266?was significantly increased?P<0.05?.Compared with the model group?3.3420+0.2266?,the total cholesterol content of the piperine 20 mg·kg-1 dose group?2.6509+0.4458?and the piperine 40 mg·kg-1 dose group?2.4090+0.5843?decreased significantly.Low?P<0.05?.3.Effect of piperine on triglyceride content in serum of mice with cholesterol gallstoneCompared with the model group?0.9010+0.1694?,the content of triglyceride in piperine20 mg·kg-1 dose group?1.3358+0.5908?,piperine 40 mg·kg-1 dose group?1.7409+0.5797? increased significantly?P<0.05?.4.Effect of piperine on serum high density lipoprotein cholesterol in cholesterol gallstone miceCompared with the blank control group?1.1600+0.2655?,the HDL-C content in the model group?0.7940+0.2715?decreased significantly?P<0.05?.Compared with the model group?0.7940+0.2715?,the HDL-C content in the piperine 20 mg·kg-1 dose group ?0.9900+0.1010?increased significantly?P<0.05?.5.Effects of piperine on serum low density lipoprotein cholesterol in mice with cholesterol gallstoneCompared with the blank control group?0.1211+0.03919?,the LDL-C content in the serum of model group?0.2489+0.08609?increased significantly?P<0.05?.6.Effect of piperine on serum alanine aminotransferase in mice with cholesterol gallstoneCompared with the blank control group?44.5555+9.8502?,the ALT content in the serum of model group?133.0000+54.6626?increased significantly?P<0.05?.Compared with model group?133.0000+54.6626?,the ALT content in serum of 20 mg·kg-11 group?83.7000+24.7971?decreased significantly?P<0.05?.7.Effect of piperine on total bile acids in serum of mice with cholesterol gallstoneAll the mice in each group had no statistical significance,but there was a trend of action.From the point of view of action,the total bile acid content in the serum of the model mice was higher than that of the blank control group.Compared with model group ?11.4222+11.4880?,the concentration of total bile acids in the serum of piperine 20 mg·kg-1group?8.7200+3.6181?decreased.8.Effects of piperine on cholesterol stone formation in C57BL/6 mice induced by high-fat dietThe results of Western blot showed that the expression level of NPC1L1 protein in the intestinal mucosa epithelium of the model mice was higher and the expression of SR-BI protein was lower,and the expression level of NPC1L1 protein in the dose group of piperine20 mg·kg-1 and the dose group of piperine 40 mg·kg-11 was obviously decreased,and the expression level of SR-BI protein was obviously increased.Conclusion:1.Piperine inhibited the formation of cholesterol gallstone in C57BL/6 mice.2.Piperine inhibits the expression of NPC1L1 protein in the jejunum of C57BL/6 mice induced by high fat diet and promotes the expression of SR-BI protein.Piperine reduces the absorption of cholesterol and inhibits cholesterol gallstones.3.Inhibiting the absorption of NPC1L1 and SR-BI protein on the intestinal jejunum and inhibiting the formation of cholesterol gallstones in the gallbladder in mice is one of the mechanisms of the inhibition of the cholesterol stones of piperine. |
PDF Full Text Request