The Characteristics Of AMCI TCM Syndromes And Their Correlation With Serum IL-6, CCL2 And MIF

Purpose:The purpose of this study was to investigate the patterns of TCM syndrome types and distribution characteristics in amnestic mild cognitive impairment(aMCI),To detect levels of interleukin-6(IL-6),Chemokine CC motif 2(also known as monocyte chemoattractant protein-1),and macrophage migration inhibitory factor(MIF)levels in serum of aMCI,to investigate the correlation of serum IL-6,CCL2,and MIF levels with aMCI.Material and method:From December 2016 to March 2018,600 cases of resident Han subjects were collected from Liaoning University of Traditional Chinese Medicine Affiliated Hospital and Rehabilitation Center,General Hospital of Shenyang Military Region,and Liujia Community Retirement Center.300 patients with aMCI were selected.Cases,300 old people with normal cognitive function matched with their age and gender.Subjects were collected for general clinical data,and used the "Chinese medicine four diagnostic information collection form",the Mini-Mental State Examination Scale(MMSE),Hamilton Anxiety Scale(HAMD),Ischemia Index(HIS),and clinical The Dementia Rating Scale(CDR)and the Daily Living Capacity Scale(ADL)were used to collect TCM syndromes and assess cognitive function.The levels of IL-6,CCL2,and MIF in serum were determined by enzyme-linked immunosorbent assay(ELISA)and their association with aMCI was explored.By sorting and summarizing the information of four Chinese medicines for 300 aMCI patients who meet the requirements,the Latent class analysis(LCA)was used to summarize the syndromes of Chinese medicine syndromes of aMCI,so as to find out the six main certificates of aMCI optimization and stability.Type,and to explore the association of aMCI TCM syndrome type with serum IL-6,CCL2,and MIF levels.Results:1.The statistics found that there was no statistical difference in age and gender between the aMCI group and the cognitive normal group.The education level of the aMCI group was lower than that of the cognitive normal group(P<0.05),and both the depression score and the viability score were lower than In the normal control group(P<0.05),smoking and alcohol consumption were higher in the aMCI group than in the cognitive normal group(P<0.05).2.Analysis of the collected information of the four Chinese medicines of 300 patients with aMCI found that the frequency of the top 15 symptoms are: forgetfulness,diminished intelligence,unresponsiveness,dizziness,fatigue,weak waist,miscalculation,fatigue,frequent urination,dry stool,chest tightness,tinnitus,shortness of breath,upset,dry mouth.After the analysis of latent variables,the six most stable and optimized syndromes were obtained,corresponding to liver and kidney deficiency syndrome,liver qi stagnation syndrome,phlegm turbid phlegm syndrome,spleen and kidney yang deficiency syndrome,heart and spleen deficiency syndrome,and kidney essence deficiency.certificate.3.The serum levels of IL-6 in the aMCI group were higher than those in the normal group.There was a significant difference between the two groups(P<0.05).The serum CCL2 level in the aMCI group was higher than that in the cognitive normal group,and the difference between the two groups was statistically significant(P<0.05).The serum MIF level in the aMCI group was higher than that in the normal cognitive group.There was a statistically significant difference between the two groups(P<0.05).4.The analysis found that the level of IL-6 in the aMCI turbid phlegm and blood stasis syndrome was significantly higher than other groups,and there was a statistically significant difference with other syndromes(P<0.05),followed by liver qi stagnation,and other syndromes existed Significant statistical differences(P < 0.05),no significant differences between the remaining syndromes.The serum level of CCL2 in aMCI spleen and kidney yang deficiency was the highest,which was significantly different from other syndromes(P<0.01).There was no significant difference between the remaining syndromes.There was no significant difference in serum MIF levels between aMCI syndromes.Conclusion:1.The age and sex of the two groups matched,and the proportion of aMCI patients in education was relatively low.The aMCI patients were more prone to depression and other undesirable emotions,and their living ability was significantly lower than that of cognitive normal subjects,and smoking and drinking were higher.2.The top 15 symptoms of aMCI are: forgetfulness,loss of intelligence,dull expression,dizziness,weakness,lassitude,miscalculation,divine fatigue,urinary frequency,dry stool,chest tightness,tinnitus,shortness of breath,upsetness,and dry mouth.Forgetfulness and intelligence are the most frequent.The most common syndromes in patients with aMCI were liver and kidney deficiency syndrome,followed by heart and spleen deficiency syndrome,phlegm and cloudiness syndrome,liver qi stagnation syndrome,spleen and kidney yang deficiency syndrome,and kidney essence deficiency syndrome.3.Serum IL-6,CCL2,and MIF levels play an important role in the development of aMCI.Serum IL-6,CCL2,and MIF levels provide a reference for the diagnosis of aMCI.4.Serum IL-6 levels were significantly correlated with aMCI turbid phlegm syndrome and liver qi stagnation syndrome.There was a significant correlation between serum CCL2 level and aMCI spleen kidney yang deficiency syndrome.There was no correlation between serum MIF levels and aMCI TCM syndromes.