Correlation Study Of AMCI TCM Syndromes With COX-2, CD33 Serum Levels And Gene Polymorphisms

Purpose:In this study,based on the underlying analysis method,data mining was conducted on the syndromes of aMCI and the syndrome elements.This is study that examines the correlation between the serum levels and genetic polymorphism of the Amnestic Mild cognitive impairment,which is based on the serum levels and genetic polymorphism of the Cluster of enzymes in the cyclooxygenes-2,and the cell differentiation antigen 33.The correlation between aMCI syndrome and COX-2 and CD33 and gene polymorphism was discussed.Material and method:1.In accordance with the principle of case-control study,600 cases of han Chinese subjects were collected in 2 grade 3 grade a hospitals and 6 nursing centers in shenyang,among which300 cases of aMCI group and 300 cases of cognitive normal group which has the same age and gender with aMCI group.General clinical data collection,the history of all subjects on acquisition and mental nerve scale detection.Through sorting out and summarizing the four TCM consultation information of 300 aMCI patients,potential category data analysis method is applied to induction syndromes law of aMCI,thus find out the six major aMCI optimization and stable card type.2.The serum COX-2and CD33 protein levels were detected by enzyme-linked immunosorbent assay?ELISA?,and their correlation with aMCI was discussed.Obtain the information and sequence of COX-2 and CD33 through Gen Bank of the national biological information center.Using multiple high-temperature link enzymes to detect the genetic polymorphisms of the COX-2?rs689466 rs20417?and CD33?rs3865444?in the blood of the aMCI patients,to analyze the genetic polymorphism of the aMCI population,and the allele distribution of the alleles.3.Explore wherther there is a correlation between a MCI TCM syndrome type and cox-2 and CD33 serum protein levels,gene polymorphism and the onset of aMCI.Results:1.The aMCI group's level of education is lower than that of the normal cognition(^*P<0.05,^**P<0.01);smoking status and alcohol degree are higher than that of the normal cognition(^*P<0.05,^**P<0.01);and depression scores and life ability score are lower than that of normal cognition(*P<0.05,^**P<0.01).2.In comparison with the normal group,aMCI CD33,cox-2 serum level is significantly higher than that of normal group?^*P<0.05?;the aMCI group cox-2 gene-rs689466 polymorphism is no statistical difference?P>0.05?;COX-2-rs20417-G/G,COX-2-rs20417-G/C,COX-2-rs20417C/C genotype is statistically significant^?*?P<0.05;COX-2-rs20417-G,COX-2-rs20417-C allele is statistically significant?^*P<0.05?;There were statistically significant differences in the rs3865444CC,rs3865444C/A?rs3865444A/A genotypes in the aMCI group of CD33^?*?P<0.05;and there were statistically significant differences in rs3865444C and rs3865444A alleles?^*P<0.05?.3.According to the analysis of the collected data of the 300 patients with aMCI,the first 15symptoms were:good memory,mental decline,slow response,dizziness,fatigue,weak waist and knee,loss of calculation,weakness,frequent urination,dry stool,chest tightness,tingling,shortness of breath,irritability and dry mouth.Through the analysis of the latent variables,the six most stable and optimized syndromes were obtained,which were respectively corresponding to deficiency of liver and kidney,deficiency of heart and spleen,obstruction of phlegm,qi stagnation of liver,Yang deficiency of spleen and kidney,deficiency of kidney essence.4.COX-2 and CD33 protein levels showed the highest expression level in turbid sputum syndrome with statistically significant differences?a:P<0.05?,followed by liver and kidney deficiency syndrome?b:P<0.05?.CD33rs3865444C/C genotype was positively correlated with sputum turbidity and mongolism syndrome?OR=2.49,*P<0.1?.The CD33rs3865444A allele was positively correlated with sputum turbidity and mongolism syndrome ?OR=6.11,*P<0.1?.Cox-2 rs20417G allele was positively correlated with deficiency syndrome of liver and kidney?OR=8.6,*P<0.1?.Cox-2 rs689466 gene polymorphism and TCM syndrome type had no statistical significance.Conclusion:1.In aMCI patients,the expression of inflammatory factors was abnormal,and the serum levels of CD33 and cox-2 were significantly higher than that in the cognitive normal group,where the cox-2 rs20417,CD33rs3865444 gene polymorphism was associated with the pathogenesis of aMCI,COX-2 rs20417G/G,COX-2 rs20417G/C,COX-2 rs20417C/C genotype,CD33rs3865444CC,CD33rs3865444C/A?rs3865444A/A genotype was the susceptibility genotype of aMCI,COX-2rs20417G,COX-2rs20417C alleles and CD33rs3865444C,CD33rs3865444A allele is aMCI's susceptible allele.2.The symptoms of aMCI in the top 15 are:forgetfulness,loss of intelligence,sluggish response,dizziness,fatigue,weakness of the waist and knees,loss of calculation,weakness of the body,frequent urination,dry bowel movement,chest tightness,tinnitus,shortness of breath,irritation,and dry mouth.The highest frequency of forgetting and intelligent decrease;The six syndromes of aMCI were analyzed by the latent variables,which were respectively corresponding to the deficiency of liver and kidney,liver and qi stagnation syndrome,phlegm,spleen and kidney Yang deficiency syndrome,two deficiency syndrome of the heart and spleen,and deficiency of kidney essence.3.The expression of c0x-2 and CD33 protein in aMCI patients was significantly correlated with the syndrome of phlegm turbidity and osmosis and deficiency of liver and kidney,showing a positive correlation.The CD33rs3865444C/C genotype and the CD33 rs3865444A allele were positively correlated with the phlegm turbidity syndrome.Cox-2 rs20417G allele was positively correlated with deficiency syndrome of liver and kidney,while cox-2 rs689466gene polymorphism was not statistically significant with TCM syndrome type.