| Purpose : This experiment intends to establish a T2 DM rat model by diet-induced combination therapy,and study the effect of Chinese medicine Yitangkang on serum SFRP4,SFRP5,glucose and lipid metabolism,insulin resistance,etc.in T2 DM rats,aiming at T2 DM insulin resistance in Chinese medicine.From theory to intervention,the theory is based on the theory of qi and spleen and nourishing yin.From the point of view of spleen and phlegm,the traditional Chinese medicine Yitangkang is used to observe the model from multiple levels and multiple targets.The effects of SFRP4 and SFRP5 expression in rat serum and explore its mechanism of action provide theoretical and experimental basis for T2 DM treatment in Chinese medicine.In the future,clinical application can be further applied to benefit patients and reduce social burden.Materials and Methods: 50 healthy normal male Wistar rats,8 weeks old,weighing220±20 g.Rats were fed ad libitum with free diet for one week,and rats without adverse reactions were included in the formal experiment.At the beginning of the experiment,the rats were numbered according to their body weight,and then 10 random groups and 40 high-fat groups were applied by random number table method.Rats in the high-fat group were fed with high-fat and high-sugar diet,and the blank group was fed with normal diet for 4 weeks.The rats in the high-fat group were then given a small number of intraperitoneal streptozotocin(STZ)injections at a dose of 45 mg/kg body weight.The normal group was given the same amount of physiological saline buffer injection,and it was confirmed that the FPG?16.7mmol/L after 72 h,that is,the rat T2 DM model was successfully modeled.They were randomly divided into a model group,the Chinese medicine Yitangkang group,and the western medicine control metformin group.The doses of each group were converted into equivalent doses of rats for intragastric administration.The rats in the blank group were given an equal dose of normal saline.After 6 weeks of administration,the rats were sacrificed,blood was taken from the abdominal cavity and serum was separated.The levels of SERP4 and SERP5 in serum of each group were determined by enzyme-linked immunosorbent assay,and the relationship between glucose resistance and insulin resistance was observed.Results:1.Comparison found: Before the administration,the rats in the blank group were compared with the groups: the FPG was increased(P<0.05).Compared with the T2 DM group,the FPG of the Chinese medicine Yitangkang group and the metformin group showed a downward trend(P<0.01)after 4 weeks and 8 weeks of treatment.There was no statistically significant difference between the Chinese medicine Yitangkang group and the metformin group(P>0.05).2.Compared with the blank group,the TG and TC of each group increased significantly.There were statistical differences between the groups(P<0.01).Compared with the T2 DM group,the TG and TC of the Chinese medicine Yitangkang group and the metformin group were significantly lower,and there were statistical differences between the groups(P<0.01).There was no significant difference between Kang group and metformin group(P>0.05).3.Compared with the blank group,the FINS and HOMA-IR of the rats in each group were increased,and there was significant difference between the two groups(P<0.01).Compared with the T2 DM group,the Chinese medicine Yitangkang group and metformin group FINS,HOMA-IR was significantly decreased,and there was significant difference between the two groups(P<0.01).However,there was no significant difference between the Chinese medicine Yitangkang group and the metformin group(P>0.05).4.Compared with the blank group,the serum SFRP4 increased and the SFRP5 decreased in each group(P<0.01).Compared with the T2 DM group,the SFRP4 of the Chinese medicine Yikangkang group and metformin group increased SFRP5.The decline trend(P<0.01);there was no significant difference between the Chinese medicine Yitangkang group and the metformin group(P>0.05).Conclusion:1.Chinese herbal compound Yitangkang can significantly improve the glucose and lipid metabolism disorder in T2 DM rat type 2 diabetes model rats,effectively reduce FPG and improve blood lipids.2.Chinese herbal compound Yitangkang effectively acts on insulin resistance in type 2diabetes.3.Chinese herbal compound Yitangkang may affect the operation of WNT signaling pathway by regulating the changes of SFRP4 and SFRP5 proteins,so as to prevent and treat type 2diabetes. |
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