| Purpose: The effects of elemene and lobaplatin on lung adenocarcinoma cell lines A549 and H1299 were studied by observing cell morphology,proliferation inhibition rate,apoptosis and the expression of apoptotic proteins Bcl-2 and Bax.The apoptotic effects of lobaplatin and elemene on lung adenocarcinoma cell lines were discussed,so as to provide basic experimental basis for the treatment of lung adenocarcinoma patients.Material and method: Human lung adenocarcinoma cells A549 and H1299 were selected and cultured in vitro.Elemene and Loplatin were diluted and treated separately and in combination with A549 and H1299 cells at different concentrations.CCK-8 method was used to detect the inhibition rate of cell proliferation and the change of cell proliferation with the change of time and concentration.FITC-Annexin V and PI Apoptosi Kit apoptosis detection kit were used to detect the apoptosis of cells under the action of the two drugs by flow cytometry.Western blot was used to detect the expression of Bcl-2 protein and Bax protein related to apoptosis in A549 and H1299 cells treated with different concentrations of lobaplatin and elemene.Results: 1.CCK-8 results showed that elemene and lobaplatin inhibited the proliferation of lung adenocarcinoma cells A549 and H1299 respectively(P < 0.05)in a concentration-and time-dependent manner.The median inhibition concentration(IC50)of different concentrations of lobaplatin on A549 cells at 24 h,48h and 72 h were 53.021±2.708 ?mol/L,24.226±3.644 ?mol/L and 7.656±0.464 ?mol/L.The IC50 of H1299 cells at 24 h,48h and 72 h were 59.728±2.365 ?mol/L?19.569±2.280 ?mol/L?10.068±1.517 ?mol/L;The IC50 of A549 cells treated with elemene at different concentrations for 24 h and 48 h were 75.038±6.550 ?g/ml?33.518±3.130 ?g/ml,The IC50 of H1299 cells at 24 h,48h were 68.922±5.724 ?g/ml?56.394±3.688 ?g/ml.The IC50 of A549 cells treated with elemene at different concentrations for 48 h was 12.904±2.500 ?g/ml,and that of H1299 cellstreated with elemene at 48 h was 11.545±1.838 ?g/ml.The IC50 of elemene IC50 combined with different concentrations of lobaplatin was 3.426±0.355 ?g/ml for 48 h in A549 cells and 8.049±1.203 ?g/ml for 48 h in H1299 cells.The Combination Index(CI)of A549 is 0.581.The CI of H1299 is 0.700.2The IC50,which was detected by flow cytometry for 48 hours,acted on A549 and H1299 cell lines.The inhibition rates of A549 cell lines were 7.72%,45.2%,44.37%,63.1% and 82.13% respectively in blank group,traditional Chinese medicine group,western medicine group,combination group 1 and combination group 2.The inhibition rates of H1299 cell lines were 3.84%,27.05%,31.67%,42.69%,66.53%.Elemene and Lobaplatin could promote cell apoptosis,and the apoptotic rate of combined drug group was higher than that of single drug group,with statistical significance(P < 0.05).3.Western blotting assay showed that after 48 h of combined action of lobaplatin,elemene and two drugs,the expression of Bcl-2 protein decreased,while the expression of Bax protein increased,and the effect of combined group was more obvious.Conclusion: 1.Lobaplatin and elemene can inhibit the proliferation of lung adenocarcinoma A549 and H1299 cells.The inhibition rate is dependent on concentration and time.The combination of the two drugs has stronger inhibition than the single use.They have synergistic effect.2.Combination of two drugs can promote apoptosis of lung adenocarcinoma A549 and H1299 cells.3.The combination of the two drugs can promote cell apoptosis,which may be related to the decrease of Bcl-2 expression and the increase of Bax expression. |
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