Research On The Extraction Technology And Quality Standard Of Velvet Antler Polysaccharides

Purpose:1.Screening and optimizing the optimum extraction technology of pilose antler polysaccharide by ultrasound.2.Determination of sika deer velvet and antler medicine and extract polysaccharide content;And do qualitative identification and water inspection for antler antler extract.3.Compare the differences in the composition and content of monosaccharides between sika deer velvet and deer antler polysaccharides.Materials and methods:1.In this experiment,the principle of water extraction and alcohol precipitation was adopted.Water was used as extraction solvent to extract polysaccharides from velvet antler by ultrasonic extraction.The content of polysaccharides in velvet antler was determined by phenol-sulfuric acid method,and L9（3⁴）orthogonal design experiment was carried out as evaluation index.At the same time,the effects of particle size,extraction times,ratio of material to liquid and extraction time on the extraction process were studied,and the effects of degreasing on the content of polysaccharides in velvet antler were compared.By comparing the effects of extraction times on ultrasound,the optimum extraction times of ultrasound was selected to improve the extraction process of antler polysaccharide.2.After sika deer and wapiti velvet were shaved and cut into pieces,the oil ether of 30-60boiling range was used to degrease,then the crude polysaccharides of Sika Deer Velvet were extracted by water extraction and alcohol precipitation,respectively.Then the protein was removed by Sevage method and decolorized by hydrogen peroxide and qualitatively identified by Molish test（α-naphthol test）,anthraquinone-sulfuric acid test,Fehling test and UV spectroscopy,starch iodine test;Then using phenol-sulfuric acid method,D-anhydrous glucose as reference,determine the sika deer and deer antler medicine and extract polysaccharide content and its extracts for water examination and qualitative identification.3.After sika deer and wapiti velvet were shaved and cut into pieces,the oil ether of 30-60boiling range was used to degrease,then the crude polysaccharides of Sika Deer Velvet were extracted by water extraction and alcohol precipitation,respectively.Then the protein was removed by Sevage method and decolorized by hydrogen peroxide.After hydrolysis with trifluoroacetic acid and pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone reagent,the differences of monosaccharide composition and content between sika deer antler and red deer antler were analyzed by high performance liquid chromatography.Results:1.The optimum extraction technology in this experiment is as follows:the granularity of medicinal materials is 80-100 mesh,extraction three times,the ratio of liquid to material is18:1,extraction time is 40 minutes;the polysaccharide content of velvet antler after degreasing is higher than that without degreasing;ultrasonic extraction is the best for three times.2.After identification,the extracted gray matter was polysaccharide,and its polysaccharide content was in the range of 18.34-31.74 mg.g^-1and the velvet refined,the polysaccharide content of the extract was significantly higher than the content of polysaccharides in herbs,and sika deer antler medicinal herbs and their extracts polysaccharide contents were higher than the red deer antler polysaccharide contents.Through methodological investigation,the standard curve established by this method has a good linear relationship in this range,and the precision,stability,repeatability and sample recovery are all in line with the requirements.Ultraviolet spectrophotometry was established to determine the content of pilose antler polysaccharides.It can be considered to be applied to the quality control of pilose antler polysaccharides.3.The polysaccharides from sika deer antler and wapiti antler were analyzed by high performance liquid chromatography.The results showed that the monosaccharide composition of sika deer antler and wapiti antler were the same,and they were composed of eight monosaccharides,but the proportion of polysaccharides was different.The content and yield of Polysaccharides from wapiti antler were lower than that of sika deer antler.Through methodological investigation,the standard curve established by this method has a good linear relationship in this range,and the precision,stability,repeatability and recovery of samples meet the requirements.It can be considered to apply this method to study the composition of monosaccharides in antler polysaccharides.Conclusion:1、The ultrasonic extraction technology of pilose antler polysaccharides is stable,reliable,simple and easy to operate.It is suitable for the extraction of pilose antler polysaccharides.2、The phenol-sulfuric acid method is simple,stable and accurate.It is suitable for the determination and quality control of pilose antler polysaccharides.3、The established Pre-column derivatization high performance liquid chromatography method is simple,accurate and reproducible.It is suitable for the determination of monosaccharides in pilose antler polysaccharides.