| Objective: To observe the effect of electroacupuncture at pericardial meridian acupoint on neurological deficit score,cerebral infarction volume and GFAP/SR/D-ser expression in MCAO rats,and to explore the possible mechanism of electroacupuncture at pericardial meridian acupoint in promoting the repair of nerve injury after cerebral ischemia.Methods: Random grouping method was used for experimental grouping.One hundred and twenty SPF rats were randomly divided into normal group(n=24),sham operation group(n=24)and model group(n=72).The rat model of MCAO was made according to the modified Zea-Longa thread occlusion method in the model group.After successful modeling,the rats were scored and randomly divided into model group(n=24),pericardial meridian group(n=24)and lung meridian group(n=24).The pericardial meridian group and the lung meridian group were treated with electroacupuncture once a day for 30 minutes for 3 consecutive days in the morning after successful modeling,while the normal group,sham operation group and model group were only treated with binding 30 min without electroacupuncture stimulation.Behavioral score was performed again after treatment,and the changes of neurological impairment in each group were compared.TTC staining,immunofluorescence staining,Western Blotting(WB)and high performance liquid chromatography(HPLC)were used to detect the infarct volume,the expression of GFAP,the expression of SR protein and the content of D-Ser in the brain tissue of rats in each group.Result:1.Behavioral score of rats:Before treatment:there was no significant difference between normal group and sham operation group,but compared with normal group and sham operation group: the scores of model group,pericardial meridian group and lung meridian group were significantly higher than those of normal group,indicating that the neurological function of rats was damaged significantly(P<0.05).After treatment:the behavioral score of model group was higher than that before treatment,indicating that the neurological deficit was aggravated 3 days after modeling.The scores of pericardial meridian group and lung meridian group were lower than before,and there wassignificant difference in pericardial meridian group(P<0.05),but there was no significant difference among the three groups(P>0.05).2.Cerebral infarction volume:the staining of brain tissue in normal group and sham operation group was normal,compared with normal group and sham operation group:the cerebral infarction volume in model group,pericardial meridian group and lung meridian group was significantly higher than that in model group,pericardial meridian group and lung meridian group(P<0.05).There was no significant difference among model group,pericardial meridian group and lung meridian group(P>0.05).3.Expression of GFAP in brain tissue:there was no significant difference in the expression of GFAP between the normal group and the sham operation group(P>0.05),but compared with the normal group and the sham operation group(P<0.05),the expression of GFAP in the model group was significantly increased,and compared with the model group and the lung meridian group:the expression of GFAP in the pericardial meridian group was significantly higher than that in the model group(P<0.05),and there was no significant difference in the expression of GFAP between the lung meridian group and the model group(P>0.05).4.Expression of SR in brain tissue:there was no significant difference in the expression of SR protein in brain tissue between normal group and sham operation group(P>0.05),but compared with normal group and sham operation group,the expression of SR protein increased significantly after modeling,and compared with model group(P<0.05),the expression of SR decreased in pericardial meridian group and lung meridian group(P<0.05),but there was no significant difference between pericardium meridian group and lung meridian group(P>0.05).5.The content of D-Ser in brain tissue:There was no significant difference in the expression of D-Ser between the normal group and the sham operation group(P>0.05),but compared with the normal group and the sham operation group,the concentration of D-Ser in the model group increased significantly(P<0.05),and compared with the model group,the concentration of D-Ser in the pericardial meridian group decreased(P<0.05),and the D-Ser concentration in the lung meridian group decreased,but there was no significant difference(P>0.05).There was no significant difference between pericardial meridian group and lung meridian group(P>0.05).Conclusion:1.Three days after MCAO,the neurological deficit in rats tended to be aggravated,and electroacupuncture at pericardial meridian acupoints could alleviate the neurological deficit in rats.2.Electroacupuncture at pericardial meridian acupoints can promote the expression of GFAP,the characteristic protein of AST in the brain tissue of MCAO rats,down-regulate the expression of SR protein,and inhibit the synthesis and release of D-Ser.3.Electroacupuncture at pericardial meridian points can improve the neurological deficit of rats,which may be related to the regulation of the expression of GFAP/SR/D-Ser.It is suggested that the signal pathway between GFAP/SR/D-Ser may be involved in the injury and repair of neurological function after cerebral ischemia. |
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