| Many studies have shown that probiotics have the functions of regulating intestinal microbiota,improving immunity,and lowering fat.However,most of the researches were conducted via probitotics intervening after the establishment of high-fat obesity models.The research on interventions in the process of establishing high-fat models is just beginning.Therefore,the intervention of Lactobacillus plantarum was conducted on mice with high-fat diet during and after modeling.It is necessary to find a way to effectively supplement probiotics to reduce fat.In order to protect Lactobacillus plantarum from gastric acid,bile salts,etc.,and reach the colon smoothly,this topic uses pectin as the microcapsule wall material to make Lactobacillus plantarum microcapsules so that it can reach the colon smoothly.The large group,the relief group and the prevention group,that is,the intervention after the establishment of the high-fat model and the intervention during the establishment of the high-fat model,the relief component is seven groups,and the prevention component is four groups.The alleviating group received intragastric administration of Lactobacillus plantarum microcapsules at the 8th w until the 12 th w;the prevention group began intragastric administration of Lactobacillus plantarum microcapsules at the 1st w to the 8th w.After the experiment,the body weight,body length,liver and spleen index of the mice were measured.The levels of immune factors and lipid metabolism cytokines in the serum were detected by ELISA,and the 16 sr DNA high-throughput sequencing platform was used at the 12 th and 8th experiments Fecal microbial genomes are analyzed.The experimental results of the alleviating group showed that the intragastric administration of Lactobacillus plantarum microcapsules could restore the weight of the mice to 28.93 ± 1.27 g,the Lee 's index was 349.1 ± 4.83,and the mice 's fecal shortchain fatty acid The content of medium acetic acid was increased to 122.83 ± 30.14 mg/L,which restored the concentration of cytokine LPS in mouse serum to 4.51 ± 0.44 EU/L,TNF-? concentration 76.52 ± 6.39 pg/m L,and IL-6 concentration 26.06 ± 4.58 pg/m L,TC concentration 0.95 ± 0.12 mmo L/L,TG concentration 276.00 ± 17.23 nmo L/L,VLDL concentration 2.34 ± 0.24 mmo L/L,IL-22 concentration 6.62 ± 0.52 pg/m L,HDL concentration 20.56 ± 2.10 mg/d L,LEP Concentration 1.38 ± 0.12 pg/m L,high-fat feed will cause intestinal flora disorder in mice,reduce the ratio of Bacteroides phylum and thick-walled phylum to increase the number of harmful bacteria,Lactobacillus plantarum microcapsules can effectively increase the dominant bacteria in the intestine Relative abundance in the tract;the experimental results of the prevention group showed that the intragastric Lactobacillus plantarum microcapsule interference did not significantly change the body weight of the mouse compared with the normal group(A)group of mice 29.22 ± 1.25 g,its Lee's index was 354.29 ± 7.38,mice The fecal short-chain fatty acid has an acetic acid content of 99.92 ± 27.94 mg/L and a propionic acid content of 19.30 ± 6.20 mg/L,butyric acid content 22.49 ± 5.73 mg/L,mouse serum LPS concentration 7.11 ± 0.53 EU/L,TNF-? concentration 87.65 ± 10.01 pg/m L,IL-6 concentration 34.36 ± 4.32 pg/m L,TC concentration 0.96 ± 0.11 mmo L/L,TG concentration 290.82 ± 15.12 nmo L/L,VLDL concentration 1.84 ± 0.37 mmo L/L,IL-22 concentration 6.42 ± 0.74 pg/m L,HDL concentration 18.93 ± 1.62 mg/The concentration of d L and LEP was 1.27 ± 0.09 pg/m L.Lactobacillus plantarum microcapsules reduced the proportion of thick-walled bacteria,increased the relative abundance of dominant bacteria in the intestine,and improved the structure of intestinal flora.It will provide a more direct theoretical support for the scientific explanation of the research on the regulation of probiotics on obesity. |
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