Research And Application Of Digital Reference Materials In The Overall Quality Control Of Boswellia Serrata

Frankincense is a resin exuded from the bark of Boswellia sacra Flück.(B.carteri Birdw.)and the same species B.papyrifera(Del.)Hochst.And frankincense has good medicinal value.The existing quality standards lacks specific detection methods and controls a little components,which is insufficient to embody its holistic quality.Therefore,we discuss the holistic quality control of frankincense by controlling multi-components.However,multi-components quality control requires multiple monomer reference standards.But some components have poor stability,so it is difficult to prepare monomer reference standards and expensive.New methods should be sought for the analysis and detection.This research mainly includes the following parts:Part ? is the holistic quality control of frankincense by TLC identification,impurity test,UPLC fingerprint analysis and contents determination.Firstly,TLC identification method of frankincense was established to distinguish two types of medicinal frankincense herbs using 15 batches of Ethiopia frankincense and 10 batches of Somalilnds frankincense.Secondly,a specific impurity test method of frankincense was established by investigating the sampling method,extraction solvent,solvent volume and extraction times.Then,the UPLC fingerprint of frankincense was established,and the transfer of the fingerprint on different columns was discussed.The UPLC fingerprints of 5 batches frankincense on 10 different columns were analyzed with Chem Pattern software and 5 batches frankincense could be identified with similarity analysis,cluster analysis and principal component analysis.It was proved that UPLC fingerprint of frankincense can be used on different columns.Finally,the HPLC method and UPLC method were established to determine the content of boswellic acids in frankincense.Part ? is the identification of peaks in the quality control of frankincense by digital reference standard.Firstly,PDA spectrum assisted relative retention time(RRT)method was established to intelligent identify KBA and AKBA by Digital Reference Standard software(DRS ORIGIN).And this method was used to identify KBA and AKBA of frankincense in “unknown” columns.Secondly,to intelligent identify six boswellic acids of frankincense,RRT method,linear calibration using two reference substances(LCTRs)method,PDA spectrum assisted RRT method and PDA spectrum assisted LCTRs method were established by DRS ORIGIN.Four methods were compared and identified six boswellic acids of frankincense in “unknown” columnsby the optimal method.The results showed that,except for the KBA on Col 7,which was not recognized,the peaks on the remaining columns were correctly identified.Part ? is the quantitative study of peaks in the quality control of frankincense by digital reference standard.Relative correction factors were used to calculate the content of boswellic acids of frankincense.Firstly,AKBA was taken as the reference standard to obtain the relative correction factor of KBA and the key factors,such as detection wavelength,PH value and the ratio of mobile phase affecting the relative correction factor were investigated.The content of KBA and AKBA in 25 batches frankincense was determined by the relative correction factor and the results were no significant difference from the external standard method.Secondly,AKBA and?-ABA were taken as the reference standards to obtain the relative correction factor of the other four boswellic acids and influence of detection wavelength on relative correction factor was investigated.The content of six boswellic acids in 25 batches frankincense was determined by the relative correction factor and the results were no significant difference from the external standard method.Finally,to evaluate the reliability of the relative correction factor measurement,we analyzed the sources of uncertainty,evaluated uncertainty of each component and synthesized the relative standard uncertainty.The results showed that the mass(purity and weighing)of the reference standards had the greatest influence on the relative correction factor measurement,followed by the preparation of the mixed reference standards solution,and other factors had less influence.