Study On The Effect And Mechanism Of Ginger/6-gingerol In Improving Glucose And Lipid Metabolism Disorders In Rats

Objective To investigate the effects and mechanism of ginger on liquid fructose-induced skeletal muscle insulin resistance.Methods 24 male SD rats were randomized into normal group,model group,low and high dose of alcoholic extract of ginger treatment group(20 mg/kg,50 mg/kg ig.)For 4 weeks;Insulin sensitivity index(ISI),homeostasis model assessment of insulin resistance(HOMA-IR)was detected and calculated,non-esterified fatty acid(NEFA/FFA)was detected;RT-PCR and Western blot were used to detect the expression of mRNA and protein.Results Ginger treatment reversed the liquid fructose-induced low ISI and high HOMA-IR,decreased NEFA(P < 0.05),increased the PPAR? mRNA expression(P < 0.01),but had not alert the expression of PI3 K,IRS-1,Akt,CD36,GLUT4,PGC1? and CPT1? significantly,moreover,ginger treatment improved the protein expression of CD36,PPAR? and the ratio of p-Akt/Akt(P < 0.05).Conclusion Alcoholic extract of ginger treatment can improve fructose-induced skeletal muscle insulin resistance by up-regulating the pAkt/Akt,CD36 and PPAR? protein,also PPAR? mRNA.Objective To investigate the effects of 6-shogaol on age-related hepatic lipid accumulation and explorer its potential molecular targets in rats.Methods Four groups were set up in the experiment,the old male rats were assigned into the old control group(Aging),the 6-shogaol low-dose group(SL,0.125mg·kg-1),the 6-shogaol high-dose group(SH,0.5 mg·kg-1),and the young male rats were set as the young control group(Young).Gavage for 7 weeks.At the end of experiment,detecting blood biochemical indexes.liver tissues were used to determine the expression of genes and proteins related to lipid synthesis and oxidative metabolism.Results We found that 6-shogaol reduced blood plasma total cholesterol(TC),non-esterified fatty acid(NEFA),glucose(GLU),insulin,homeostasis model assessment-insulin resistance(HOMA-IR),and liver triglyceride(TG)contents,decreased sterol regulatory element-binding protein(SREBP)-1c,liver pyruvate kinase(L-PK)and fatty acid synthase(FAS)m RNA expression,increased CPT1 A m RNA expression.Furthermore,6-shogaol reduced expression of mature form of SREBP-1 protein,Ch REBP protein in nuclear,FAS and DGAT2 protein,increased expression of peroxisome proliferators-activated receptor(PPAR)?,peroxisome proliferator-activated receptor ? coactivator(PGC-1)-1?,carnitine palmitoyltransferase 1A(CPT1A)protein and also upregulated sirtuin 1(SIRT1)and AMP activated protein kinase(AMPK)activation protein level.Conclusion 6-shogaol can attenuate age-related hepatic lipid accumulation in rat,which is associated with regulating SIRT1/AMPK pathway.