| Background:In the advancement of the traditional Chinese medicine,research on the material basis of prescriptions is one of the significant aspects,and formula is generally used in the form of decoction in the clinical treatment.Due to the complexity and diversity of the chemical compositions in formula,the comprehensive analysis of constituents in vitro and in vivo are rather difficult,few research is reported on the material basis of compound,but transform their eyes on the quality control,such as the standardization of traditional Chinese medicine and fingerprint.This article selects the clinical prescription modified Suanzaoren decoction(MSD),and studies the chemical compositions of the water extract of modified Suanzaoren decoction,the components absorbed into plasma and urine metabolites after oral administration of modified Suanzaoren decoction extracts by rats.Furthermore,this research provides the experimental foundation in the development of pharmaceutical formulation and quality control of the formula.It is useful for the in-depth pharmacological research of modified Suanzaoren decoction.Objective:To study the material basis in vitro and in vivo of the modified Suanzaoren decoction.Liquid chromatography with mass spectrometry(LC-MS)was applied to analyze the chemical components of modified Suanzaoren decoction in the form of decoction.The components absorbed into plasma and the metabolic components of urine at different time points were detected after oral administration of modified Suanzaoren decoction extracts by rats.Methods:(1)The extraction solution of compound modified Suanzaoren decoction was obtained by taking water as the solvent in proportion.The chemical components of the extraction solution of modified Suanzaoren decoction were analyzed by UPLC-Q-TOF-MS in the both positive and negative ion modes,respectively.Further,the material basis of compound modified Suanzaoren decoction in vitro were identified with different methods.(2)Orbital blood samples were collected after rats were given the extract of compound modified Suanzaoren decoction by gavage,moreover,the chemical components of blank plasma and sample plasma were analyzed by UPLC-Q-TOF-MS and the components absorbed into plasma were identified with different methods.(3)Rats were given deionized water after oral administration of Modified Suanzaoren decoction extracts,with that we collected the blank urine of rats,0-12 h urine sample and 12-24 h urine sample of rats,respectively.UPLC-Q-TOF-MS was applied to analysis the blank urine sample of metabolic components and the medicated urine sample of different time points.Results:(1)Based on the literature research,compared with standard substance and the data of MS/MS,34 constituents of modified Suanzaoren decoction in the form of decoction were analyzed,the chemical constituents mainly including saponins,phenolic acids,flavonoids and etal.The chemical components mainly come from the Ziziphi Spinosae Semen(ZSS),Anemarrhenae Rhizoma(AR),Chuanxiong Rhizoma(CR),Glycyrrhizae Radix et Rhizoma(GRR)and Polygalae Radix(PR).(2)On the foundation of the material basis of modified Suanzaoren decoction in vitro,the research explored the possible bioactive components absorbed into plasma after the rats administrating modified Suanzaoren decoction extracts.5 chemical components in the plasma were detected and identified.(3)On the basis of research in vitro and components absorbed into plasma,the urine metabolic components at different time points after rats were given modified Suanzaoren decoction were studied,and 10 chemical components in the urine were detected.Conclusion:Research of the formula modified Suanzaoren decoction in vitro and in vivo shows that the chemical constituents in the compound are diverse,mainly contains saponins and flavonoids,as well as other constituents.The analysis results cover the bioactive components that play a significant role in the basic research of single traditional Chinese medicine materials,such as the saponins of fried ZSS,diphenylpyrone in AR,ketones and oligosaccharides in PR.The primary sources of chemical composition reflect the important functions of the traditional Chinese medicine in this compound,such as fried ZSS,AR,PR and GRR.After the rats orally administrated modified Suanzaoren decoction extracts,the study on the bioactive components absorbed into plasma indicated that when the formula applied in the clinical,the main functional substances in compound may take action in two pathways as the prototype components or metabolites.The functional constituents mainly come from the medicine ZSS,AR,CR or GRR.The analysis of metabolic components in urine at different time points after the rats were given MSD showed that when the compound was applied in the form of decoction,the absorbed chemical components could produce different biological transformations in the body,and then generate the therapeutic substances. |
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