Analysis Of Clinical Data Of 290 Cases Of Ulcerative Colitis And Study On The Mechanism Of Qingchang Huashi Decoction

Purpose and significance1.In this study,through the analysis of clinical data of 290 patients with ulcerative colitis in Beijing Traditional Chinese Medicine Hospital affiliated to Capital Medical University,statistically describe the epidemiology,morbidity,intestinal microflora(stool ratio)of UC stool The distribution ratio of the club ratio to the patient's gender,age,course of disease,clinical classification,TCM syndrome and other factors,in order to improve the understanding of the disease,try to provide more favorable objective quantitative indicators for the clinic,for clinical diagnosis and Treatment provides new ideas.2.In the experiment,TNBS combined with ethanol enema was used to make UC rat model.Through the effect of Qingchanghuashi Decoction on cell apoptosis and NF-KB nuclear factor p65 protein in ulcerative colitis rats,Qingchang Huashi Decoction was used to investigate ulcerative colitis Possible mechanism of action.Research methods1.Case data analysis of UC patients:Case source:The medical records of inpatients diagnosed with ulcerative colitis from August 1,2008 to December 30,2017 were selected from the medical records of the medical record room of Beijing Traditional Chinese Medicine Hospital affiliated to Capital Medical University and the electronic medical records of the hospital's HIS system.After the exclusion criteria were screened,a total of 290 medical records that met this study were selected.According to the Western medicine diagnostic criteria for ulcerative colitis established by the Consensus Opinions on Integrated Ulcerative Colitis(2017),the medical records of the screened patients are diagnosed and classified clinically,classified,and classified by Western medicine;according to the Montreal classification method Sort out the disease scope of the medical record data;and record the patient's age,sex,course of disease,main clinical symptoms,diagnosis of traditional Chinese and Western medicine,stool routine,stool ratio,Western medicine treatment,Chinese medicine prescription usage.Use Microsoft Excel 2010 to establish a database,use SPSS20.0 for statistical analysis,and study the distribution of UC club-to-club ratio.2.UC Rat Experiment:48 male SD rats were randomly divided into 6 groups by digital table method,each group of 8 rats,each group was:normal control group(ZC group),model group(MX group),high-dose group of Qingchang Huashi Tang(GJLQ group),Qingchanghuashitang middle-dose group(ZJLQ group),Qingchanghuashitang low-dose group(DJLQ group),sulfasalazine group(SASP group).Except for the ZC group,the remaining 5 groups used TNBS combined with ethanol enema to make UC rat models.After successful modeling,each group is divided into distilled water,distilled water,high-dose Qingqinghuashitang(3.8g/ml),medium-dose Qingqinghuashitang(1.9g/ml),low-dose Qingqinghuashi decoction(0.95g/ml),sulfasalazine(mass concentration of 0.1g/ml solution),the administration volume is 10ml/Kg.The medicine was given by intragastric administration once a day for 12 days.24 hours after the last administration,the rats were sacrificed,and the anus to the cecum colon(approximately 8 cm)were selected,cut along the longitudinal axis of the mesentery,fixed with 4%formaldehyde,embedded in paraffin,4 ?m serial sections,followed by UC rat intestinal mucosal cell withering Death detection and NF-?B p65 immunohistochemical staining experiment.Quantitative data is described by the mean�standard deviation(x�s),and the comparison between groups is analyzed by analysis of variance,and further comparison between two groups.If the variances are all tested by LSD;the test level a is set to 0.05 unless otherwise specified.P<0.05 is considered to be a significant difference,and serves as a statistically significant criterion.Research resultsI.UC patient case data analysis:1.A total of 290 cases were included in this study,including 158 males(54%)and 132 females(46%);the age of onset was 17-83 years old,with an average age of 45.17 �15.38years old.The age ranged from 50 to 60 years old;patients mostly engage in mental work.2.The frequency of the patient's cue ratio from high to low is:30%:70%,40%:60%,only a small amount of cocci are seen under the microscope,20%:80%,50%:50%,60%:40%,70%:30%,80%:20%,10%:90%,90%:10%.3.The frequency of TCM syndrome types from high to low is as follows:large intestine damp-heat syndrome>spleen deficiency dampness syndrome>cold and heat miscellaneous syndrome>spleen deficiency yang deficiency syndrome>liver depression and spleen deficiency syndrome>Yin blood deficiency syndrome.4.The frequency of internal medicine prescriptions from more to less is:Shaoyao Decoction>Shenling Baizhu San>Wumei Pills>Pulsatilla Soup,Tongxie Yaofang>Lizhong Decoction>Sishen Pills>Gegen Qinlian Decoction>Si Ni San>Four Gentlemen's Soup>Parking Pills>Aconite Lizhong Soup.5.Most of the cases included in this study are active cases,mainly of chronic recurrence type,the severity is mostly moderate,and the lesions mostly involve the left colon.Western medicine often treats mesalazine by mouth,sulfasalazine to embolize the anus,oral peficon,and intestinal health.6.The UC stool club ratio in this research is significantly different from the patient's age,disease course,stool traits,and TCM syndrome types(P<0.05);there is no significant difference from gender,clinical type,and disease stage(P>0.05).?.UC Rat Experiment1.Apoptosis of rat colon cells:Compared with the ZC group,the apoptosis of the colon cells in the MX group increased significantly,and the difference was statistically significant(P<0.05);compared with the MX group,the GJLQ group,ZJLQ group,DJLQ The difference between group and SASP group was significant(P<0.05).2.Immunohistochemical staining of NF-KB nuclear factor p65 protein:Compared with the ZC group,the NF-K B nuclear factor p65 protein expression in the MX group of rats was significantly increased(P=0.002);compared with the MX group,the ZJLQ group The expression of NF-KB nuclear factor p65 protein in the colon of rats in the DJLQ group was significantly different(P<0.05).The expression of NF-KB nuclear factor p65 protein in the colon of the GJLQ group and SASP group was not statistically significant(P>0.05).Conclusion?.Clinical data analysis of UC patients1.There is no obvious relationship between the onset of the patient and gender.The inpatients are engaged in mental work more than physical work.2.The TCM syndromes mainly focus on the large intestine damp-heat syndrome and the spleen-deficiency dampness syndrome.Clinically,Shaoyao Decoction and Shenling Baizhu Powder are commonly used for treatment.3.Cases with chronic recurrence are the most common,and the severity is mostly moderate,and the lesions mostly involve the left colon.4.The clinical manifestations of the patients are mainly mucus bloody stools,diarrhea,abdominal pain,and tenesmus.Most patients have different degrees of microflora disorders.The treatment is usually combined with traditional Chinese and Western medicine5.There is a correlation between the patient's age,course of disease,stool traits,traditional Chinese medicine syndrome and stool cue ratio,which can provide some new ideas for the imbalance of intestinal flora in ulcerative colitis.?.UC Rat Experiment:1.Qingchang Huashi Decoction can alleviate the apoptosis of UC rats and has a certain therapeutic effect on the disease.2.Mid-dose and low-dose Qingqing Huashi Tang can reduce the expression of NF-KB nuclear protein p65 in the colon of UC rats,inhibit the TLR-4/NF-K B pathway,and block the intestine induced by NF-K B nuclear activation Inflammation and immune disorders of mucosal cells have a certain therapeutic effect on ulcerative colitis.