Hydrogen is a clean, efficient and versatile energy source which makes it a suitable alternative to fossil fuels. Mixed anaerobic cultures has the potential to produce hydrogen in a sustainable way in methanogenic bacteria can be inhibited.;The highest amount of hydrogen produced took place in cultures fed LA plus xylose, glucose and the 50%/50% sugar mixture with yields of 2.13+/-0.05, 2.46+/-0.19 and 2.32+/-0.17 mol H2/mol sugar, respectively. The maximum yields generated on a mol hydrogen per mass of sugar was 13.65, 14.20 and 14.08 mmol H2/g sugar for the respective sugars fermented. The final results showed that the ratio of the two different sugars did not have a significant difference in the hydrogen yield.;Batch studies were performed to assess the fermentation of glucose and xylose individually and together to observe if the sugar mixture is effective in hydrogen fermentation. Experiments were performed using a variety of LCFAs in order to inhibit methanogens so hydrogen can be collected. |