Exposure to polybrominated diphenyl ethers (PBDEs) suppresses the release of pro-inflammatory products by alveolar macrophages in vitro |
Posted on:2011-07-02 | Degree:M.S | Type:Thesis |
University:University of New Hampshire | Candidate:Hennigar, Stephen R | Full Text:PDF |
GTID:2441390002969124 | Subject:Environmental Health |
Abstract/Summary: | |
Endocrine disrupting chemicals have adverse effects on immune function that may result in respiratory conditions. Inhalation of dust is a major route of exposure to PBDEs; however, the impact of PBDEs on the immune response is unclear. The objective of this in vitro study was to determine the impact of PBDEs on the release of pro-inflammatory cytokines by activated alveolar macrophages. Porcine alveolar macrophages were grown in RPMI growth media supplemented with 10% porcine serum and incubated for 24-hours. After 24-hours, cells were activated by inoculation with PMA. In addition to PMA, different concentrations of the PBDE mixture DE-71 were introduced to the wells. After 6-hour incubation, conditioned media was removed and analyzed. Cells exposed to PMA and PBDEs released significantly less pro-inflammatory cytokines compared to controls. Suppression of pro-inflammatory cytokines—characteristic of a compromised immune system—suggests that persistent exposure to PBDEs may increase the susceptibility to respiratory conditions. |
Keywords/Search Tags: | Pbdes, Pro-inflammatory, Alveolar macrophages, Exposure, Immune |
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