| Citrus tatter leaf virus (CTLV) is a filamentous capillovirus that causes bud union crease on trifoliate hybrid rootstock. CTLV seed transmission in Lilium longiforum and Chenopodium quinoa was previously reported. However in citrus, CTLV is readily transmitted mechanically. The presence of the virus was confirmed by PCR as well as with biological indexing in four field grown citrus trees; Meyer lemon, Clementine mandarin, Eureka lemon, and Meiwa kumquat. Seeds collected from CTLV-positive trees were grown and the seedlings were tested by RT-PCR for presence of the virus. A total of 355 seedlings were tested by PCR, and two Eureka lemons were found to be CTLV positive. None of the other species produced positive results. DNA Sequence information from CTLV positive Eureka lemon seedlings displayed more homology with CTLV from Meyer lemon than the Eureka lemon field tree. Four months after inoculation with infected Eureka lemon buds, C22 rootstock plants showed leaf distortion and zigzagging stems. Furthermore, CTLV presence in C22 rootstock was confirmed by PCR. |
