Regulation of lipoprotein lipase and glucose uptake in cardiomyocytes involves RhoA/ROCK/Cofilin-dependent actin cytoskeleton remodeling

Obesity increases the risk of heart failure and has been correlated with changes in adipokine secretion profile. Adiponectin has been shown to be down-regulated in the obese model and studies have shown in vitro and in vivo that adiponectin exerts insulin mimetic as well as cardioprotective effects in the heart. In the present study, we investigated if changes in the actin cytoskeleton were correlated with LPL activity and glucose uptake to possibly mediate metabolic activity in the heart. We found that indeed short term adiponectin stimulated actin stress fiber formation and correlated this effect with LPL activity in adult rat cardiomyocytes and glucose uptake in neonatal cardiomyocytes. In summary it was found that adiponectin stimulated actin stress fiber formation in the heart regulates both LPL activity and glucose uptake and these findings may provide insight in understanding the development of heart failure in obesity.