| Implant surface microtopography has been seen to influence bone-to-implant contact and the strength of that interface. Though angiogenesis is necessary for osteogenesis, little attention has been paid to this interrelationship. To further analyze these processes a BaSO4 radio-opaque vascular perfusion compound was developed to allow for simultaneous, non-destructive, 3D, qualitative, and quantitative analysis of bone and vasculature using MicroCT. Early time points of 2, 3, 4, 6 and 15 days were analyzed with a combination of BaSO4 vascular perfusion, MicroCT, BSEI, and/or block histology around dual surface gap healing implants in the rat femur. The BaSO 4 compound developed was found to have sufficient radio-density and perfusability to allow for simultaneous MicroCT and BSEI analysis of both bone and vasculature and block histology, of 6 and 15 day samples, demonstrated that implant surface texture does influence peri-implant osteogenesis, but at a level undetectable by the MicroCT and BSEI methods used. |
