| Glucose, the simplest and most abundant sugar in nature, is the preferred carbon source for many organisms. In the presence of glucose, genes that are essential to metabolize alternative carbon sources are repressed, a phenomenon known as carbon catabolite repression. In yeast, the Snf1p has been shown to play a central role in carbon catabolite repression and causes derepression of the genes involved in alternate carbon source utilization, by phosphorylating Mig1p. During this work, a SNF1 homolog, snfA1, has been cloned and characterized in the filamentous fungus Aspergillus nidulans. Absence of pectinase enzyme activity in culture filtrates of DeltasnfA1 strain grown on pectin, further supports our hypothesis that snfA1 is involved in the regulation of the genes encoding cell-wall degrading enzymes. Further, in order to discover the other genes being regulated by snfA1 apart from glucose-repressed genes, microarray technology has been used to compare the expression profiles of DeltasnfA1 and wild type strains grown on glucose and pectin. Our data shows that snfA1 also regulates genes involved in other cellular processes like lipid synthesis, sterol metabolism, polysaccharide metabolism, stress and transcriptional and translational regulation. Taken together, these data suggest that snfA1 of Aspergillus nidulans is involved in carbon catabolite derepression and its deletion prevents the fungus from properly metabolizing the alternate carbon sources in the absence of glucose. (Abstract shortened by UMI.)... |
