| Biological sequestration of carbon dioxide is one of the proposed methods to reduce concentration of carbon dioxide in the atmosphere. In this method, CO2 is converted to a mineral such as calcium or magnesium carbonate. An enzyme is used to promote the hydration reaction of CO2 and precipitation of the mineral. Enzyme carbonic anhydrase is known as the suitable enzyme for this purpose.;The concentration of enzyme did not impact the precipitation; however temperature was a factor and at higher temperatures less calcium carbonate was formed. Also, when the enzyme was present in the solution, calcium carbonate settled down faster. The pH did not influence the enzyme's activity, but it affected the formation of calcium carbonate.;As one aspect of the present research, a mathematical model was developed to get an understanding of the precipitation of CO2 in a reservoir. The outcome of the model was in a close agreement with the experimental results, which introduce the model as a reliable source for future estimations.;In the present work, the effect of bovine carbonic anhydrase on the hydration of carbon dioxide and also its precipitation in the form of calcium carbonate was studied through various experiments. The change in the rate of hydration reaction was measured indirectly, by measuring the change in pH of the aqueous solution. The results of the experiments showed that bovine carbonic anhydrase enhanced the hydration reaction. The effect of enzyme concentration, as well as temperature, on the hydration reaction was studied. The rate of pH drop increased with both the enzyme concentration and temperature. The precipitation of calcium carbonate was enhanced in the presence of the enzyme. |
