Developing a Singlet Oxygen Responsive Molecular Beacon Based on a Binuclear Ruthenium (II) Polypyridyl Complex | Posted on:2014-05-15 | Degree:M.S | Type:Thesis | University:State University of New York at Buffalo | Candidate:Lou, Kun | Full Text:PDF | GTID:2451390005991149 | Subject:Engineering | Abstract/Summary: | | As a developed fluorescent reporter, molecular beacons have been applied to various situations for biological detection via fluorescence quenching/restoration. Photodynamic molecular beacons generate singlet oxygen in response to stimuli. A simple DNA molecular beacon conjugated with two Ruthenium compound was demonstrated to have 3-fold fluorescence increase. In order to develop a new photodynamic molecular beacon detection mechanism, a new singlet-oxygen sensing beacon was synthesized based on a novel binuclear Ruthenium compound linked with a singlet oxygen labile carbon-carbon double bond. Synthesis was confirmed by NMR, mass spectroscopy and absorption spectroscopy. An approximate 5∼6 fold quenching was observed compared to monomericic Ruthenium complex in aqueous but not in methanol. Singlet oxygen generated from another longer wavelength photosensitizer (methylene blue) was able to restore ∼4-fold emission in the binuclear Ruthenium complex. | Keywords/Search Tags: | Molecular beacon, Binuclear ruthenium, Singlet oxygen | | Related items |
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