| A new drug delivery system based on a poly(beta-amino acid) has been synthesized from 2(5H)-furanone and N, N-dimethylethylene diamine by a simple, one step polymerization. The resulting polymer was purified by Bio Gelp-P60 size exclusion chromatography and auto separated into high, medium and low molecular weight fractions. The molecular weight distribution was controlled by adjusting the reaction temperate and reaction time. The drug delivery properties of the polymers were evaluated by studying the transfer of the anticancer drug doxorubicin to mouse embryonic fibroblasts cells and the transfer of telomerase antisense RNA (with sequence GCG CGG GGA AAA GCA) and a 4.2 kb plasmid containing the green fluorescent protein gene into the same cells. Best results were obtained using the high molecular weight polymer fraction. This result proves that this polymer is a very promising delivery system which can be used for those anticancer drugs with serious side effects and also successfully bring gene or plasmid which is hardly enter the cells by themselves before. |
