Reproductive toxicity and bioavailability of arsenic in contaminated artificial and natural soils using the earthworm (Eisenia andrei)

High concentrations of arsenic are found near gold-mine tailings. The most common form of arsenic found in soil is arsenate, which is a known toxicant. We used the standardised earthworm reproduction test for the species Eisenia andrei (E. andrei) to study the toxicity and bioavailability of arsenic-contaminated soil. Arsenic is toxic to earthworms as indicated by the decrease in survival and reproduction. Arsenic-spiked artificial soil was more toxic than arsenic-spiked field soil based on total arsenic concentration in soil. Moreover, soil from near mine tailings showed a reduced toxic effect despite its high soil arsenic concentration as compared to spiked field soil. Measurements of arsenic tissue concentrations in the earthworm indicated that uptake of arsenic into earthworm tissue was higher in spiked artificial soil as compared to spiked field soil and that the maximal body burden was 396 mug As/g dry tissue weight. However, when considering tissue arsenic concentration, spiked field soil is more toxic than spiked artificial soil. Therefore the tissue rather than soil content may better reflect the magnitude of arsenic toxicity to E. andrei.