| The conservation of aquatic biodiversity is largely dependent on the ability of researchers and managers to identify vulnerable species and prioritize conservation actions. Stream fish are facing tremendous threats due to habitat degradation, stream network fragmentation, and climate change. For this project two vulnerability indices were developed to quantify the relative vulnerability of stream fish in Missouri. These indices allowed us to identify the most vulnerable stream fish in Missouri, and also to compare how the use of traits differed from the use of measured species responses in quantifying vulnerability. The most valuable areas for stream fish conservation within and complementary to Missouri's conservation networks were identified through the use of a systematic conservation planning approach. Species representation, weighting based on vulnerability and listing status, and species-specific responses to upstream habitat degradation were utilized to identify priority areas with the conservation planning tool Zonation. This information can assist managers in identifying the species most in need of conservation and where the best opportunities exist for taking management actions. The frameworks for assessing both vulnerability and conservation value can be used or adapted for addressing similar concerns in other regions. |