| A continuous flow bioassay system was designed and constructed at a northwestern Ontario integrated pulp and paper mill. This system was employed to determine whether anal fin modification of mosquitofish (Gambusia afinis afinis ) would result from exposure to treated mill effluents. No effects on males or females were observed after exposure to 15% or 100% effluents for five months. Females exposed to control hormones did exhibit some effects.;The loading and variability of chemical oxygen demand (COD), biochemical oxygen demand (BOD5), colour, total suspended solids (TSS), resin and fatty acids (RFAs), and sterols was determined for more than sixty untreated process effluents and treated effluents over a five month period. Major contributors to effluent loading were the effluent from power boiler wet sluicing and the effluents related to the kraft bleaching. A toluene reflux extraction method demonstrated an improved recovery of sterols versus a methyl-tert -butyl ether extraction. |
