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Indirect photometric detection for the determination of captopril in tablet dosage forms by anion-exchange and ion-pair reversed-phase high performance liquid chromatography

Posted on:1999-02-15Degree:Ph.DType:Thesis
University:University of CincinnatiCandidate:Mirza, TahseenFull Text:PDF
The main objective of this thesis endeavor was to develop and validate an anion-exchange and an ion-pair reversed-phase high performance liquid chromatographic (HPLC) procedure using indirect photometric detection for the assay of captopril in pharmaceutical tablet dosage forms.;These methods are proposed as suitable alternatives to the tedious methods described in the USP monographs for captopril. The USP methods involve the determination of captopril and its degradation product, captopril disulfide by reversed-phase HPLC with photometric detection at a low wavelength of 220 nm. The impurity 3-mercapto-2-methylpropanoic (MMA) acid is determined by gas chromatography following a tedious derivatization procedure using hazardous reagents. Using methods proposed in this dissertation, captopril, captopril disulfide and MMA can be simultaneously analyzed at higher UV-wavelengths in a single run, thus offering significant cost-savings and improvement in specificity.;In the anion-exchange HPLC method, a low capacity Vydac;Optimum chromatographic conditions were determined by performing experiments based on the Box and Behnken 3 x 3 factorial design. The methods were validated to be sensitive, precise with the RSD (n = 6) of the peak area responses being less than 2.0% and accurate in the range of...
Keywords/Search Tags:Captopril, Photometric detection, Anion-exchange, Reversed-phase
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