| Molecular imprint polymers were prepared using dansyl-L-phenylalanine as the template. Acting as the chiral stationary phase, the molecularly imprinted polymer was evaluated based on the enantiomeric separation of D, L-dansylphenylalanine. Using a capillary liquid chromatographic technique, quantitative and qualitative changes in eluent composition and their influence on enantiomeric resolution were investigated. In addition, other experimental parameters such as temperature and flow rate were varied and their impact on chromatographic performance was determined. In all studies, chiral separation of racemic D- and L-dansylphenylalanine was achieved. Further assessment of these data led to postulated mechanisms of retention and examination of molecular recognition ability. |
