| The objectives of this study were to map both microsatellite and AFLP markers onto segregating barley populations, apply a core set of microsatellite markers to differentiate registered barley lines and to trace the inheritance of chromosomal segments based on pedigree.; A total of twenty new microsatellite markers were added to two existing Harrington x TR306 barley genetic maps. Further sources of microsatellite markers were examined, including wheat microsatellites and probing genomic DNA for repeats, but no additional microsatellite markers were identified.; By using eleven AFLP combinations, 189 polymorphic loci were identified and 138 were mapped onto an existing Shyri x Galena genetic map. The additional markers added 1469 cM to the map and a direct correlation was noticed between the number of AFLP markers added to a chromosome and the increase in chromosome length. Several different mapping experiments were conducted to determine the effects of different classes of markers on the Shyri x Galena map. Mapping of the new AFLP loci increased the overall map length regardless of the marker class excluded from the genetic map.; Two applications of microsatellite markers were applied to barley cultivars. First, a set of nine microsatellite markers, found on five chromosomes, were used to distinguish twenty registered barley cultivars. Secondly, twenty-five microsatellite markers were also screened on 12 barley cultivars and on their parental lines to trace the inheritance of chromosomal segments. |
