| Analyses of pheromone gland extract of female satin moths, Leucoma salicis, by coupled gas chromatographic-electroantennographic detection (GC-EAD) employing fused silica columns coated with DB-210, DB-23 or DB-5 revealed six antennally active compounds. (3Z,9Z )-cis-6,7-Epoxy-heneicosadiene (1) and (3Z,6Z)-cis-9,10-epoxyheneicosadiene (2) were identified. Of all four stereo selectively synthesized stereoisomers, SRSR-4 elicited the strongest antennal response, and by itself attracted male SM, suggesting that it is the major SM pheromone component. Addition of SRRS-4 to SRSR-4 at a wide ratio, as found in pheromone gland extracts, significantly enhanced attractiveness of SRSR-4, further suggesting that SRRS-4 is a second pheromone component. For pheromone-based monitoring of SM, a suboptimal but economical mixture of stereoisomers of 4 (in a 1:1:1:1 ratio) is recommended as a trap lure to obtain a correlation of captured males with the actual population density in the sampling area. For development of pheromone-based detection surveys of SM, further testing of SRSR-4, SRSR-4 plus SRRS-4 and stereoisomeric 4 is required. (Abstract shortened by UMI.). |
