| In this work, a novel synthetic approach to produce DO3A-tris-tBu-ester-N α-Fmoc-amino acids as building blocks for solid phase peptide synthesis (SPPS) was developed. Experimental conditions for the building block synthesis as well as their application in SPPS were optimized, accounting for their future large scale production and application to generate DOTA-peptide libraries. For the first time, a protein-peptide interacting system was used to determine the influence of the DOTA location within the peptide sequence on binding specificity. |
